Addgene

We narrowed to 7,585 results for: Lif;

Showing: 1021 - 1040 of 7585 results

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pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3

Plasmid

#122092

Purpose

AAV plasmid for expressing rsCaMPARI in neurons, nucleus localized

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

AAV

Tags

NLS-His and mRuby3

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-BFLF1

Plasmid

#162626

Purpose

Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFLIP37

Plasmid

#65505

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-Cit-attR1 and attR2-Cer-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCL20-pre-mGRASP

Plasmid

#173117

Purpose

Expresses CCL20 sender construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Paxillin LD

Plasmid

#186150

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D5A; D146A; D232A; D267A; D301A

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Paxillin

Plasmid

#186143

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Use

Tags

GFP 1-10

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Ptrc::RpaA D53A

Plasmid

#105229

Purpose

inducible expression of unphosphorylatable variant of RpaA (D53A)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

rpaA driven by Ptrc promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

D53 mutated to A

Promoter

ptrc

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Char-BFP2-TSERex

Plasmid

#124792

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Char-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCO489

Plasmid

#139732

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_AtuMas:NnLuz:trbcSE9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtuMAS

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-CaaX

Plasmid

#163848

Purpose

In vitro transcription of TurboID-CaaX. Parton lab clone JSU

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

CaaX

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w

Plasmid

#162481

Purpose

A bicistronic construct - FloxGal80 and UAS-mCD8GFP

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

Cre80Tom-GFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-RPL13A-FKBPx4

Plasmid

#104474

Purpose

Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

RPL13Ap (RPL13A Budding Yeast)

Use

Tags

FKBPx4-HA

Expression

Yeast

Mutation

includes the last 354 bp of RPL13A only

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pNP154

Plasmid

#106360

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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LexAop2->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87640

Purpose

For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFLIP30

Plasmid

#65499

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-AFPt9-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP32

Plasmid

#65500

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP39

Plasmid

#65507

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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