We narrowed to 1,282 results for: MS2

1. pCW57.1-MAT2A

   Plasmid

   #100521

   Purpose

   Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   MAT2A (MAT2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTight TRE

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-KDM4A

   Plasmid

   #188233

   Purpose

   Express human KDM4A in mammalian cells

   Depositor

   Young Tang

   Article

   Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

   Insert

   Lysine-specific demethylase 4A (KDM4A Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LIC-Z-Y-L

   Plasmid

   #153546

   Purpose

   this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Expression

   Mammalian

   Mutation

   with 6 Y in 3 ITAMs mutated to L

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. scFv-GCN4-DNMT3a(R887E)-DNMT3l

   Plasmid

   #154141

   Purpose

   Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   R887E

   Promoter

   SFFV

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

   Plasmid

   #154139

   Purpose

   Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
   

   Tags

   3x FLAG and tagBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. scFv-GCN4-DNMT3a-DNMT3l

   Plasmid

   #154140

   Purpose

   Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR2.1_HBB_N

   Plasmid

   #87847

   Purpose

   pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

   Depositor

   Marina Kleanthous

   Article

   Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

   Insert

   Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LIC-Z

   Plasmid

   #153543

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LIC-Z-delCry2

   Plasmid

   #153545

   Purpose

   this is the non-clustering version of LIC-Z tagged with mcherry

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   with Cry2 sequence deleted from LIC-Z

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid

    #160458

    Purpose

    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Inserts

    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    

    Use

    Insertion of a fluorescent reporter through homol…

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LIC-Z-YFP

    Plasmid

    #153544

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Tags

    Venus YFP

    Expression

    Mammalian

    Mutation

    with mCherry in LIC-Z replaced by YFP

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-Cx46-IRES-GCaMP6s

    Plasmid

    #188238

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJA3 (GJA3 Human)
    

    Use

    Lentiviral

    Tags

    GCaMp6s

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC39A4

    Plasmid

    #132069

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A4 (SLC39A4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Promoter

    osk

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-EGFPC-uL10-(Neo)R

    Plasmid

    #158069

    Purpose

    eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    RPLP0 (RPLP0 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-U6-dgRNA-CAG-MPH

    Plasmid

    #106259

    Purpose

    Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. lucMAPT-30D

    Plasmid

    #214672

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lucMAPT-30U

    Plasmid

    #214673

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57-Cx26-IRES-GCaMP6s

    Plasmid

    #188236

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    GCaMP6s

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-SLC39A4_STOP

    Plasmid

    #161235

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A4 (SLC39A4 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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