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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

Plasmid

#75132

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

Plasmid

#75129

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75123

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Mutation

bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Mutation

SET domain replaced with the SET domain of EZH1

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75120

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV N-term HA-FLAG puro BRD7 shRescue

Plasmid

#41936

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-497/miR-195

Plasmid

#64236

Purpose

Expresses miR-497 and miR-195 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-497 and miR-195 (MIR195, MIR497 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(280-342)

Plasmid

#72550

Purpose

expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

280-342 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(384-645)

Plasmid

#72549

Purpose

expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

384-645 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

Plasmid

#75121

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRG1

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

Plasmid

#75119

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD1

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV T274V S392A KSR1 IRES GFP

Plasmid

#25979

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine and serine 392 to…

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-PIG-FLAG-TAF4(827-909)

Plasmid

#105617

Purpose

retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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