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pStreptagII 3C EGFR EABR

Plasmid

#234994

Purpose

For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Publication

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

EGFR-EABR (EGFR Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-TeNT

Plasmid

#240990

Purpose

Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-TeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230989

Purpose

Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

Depositor

Adam Cohen

Publication

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

Plasmid

#234881

Purpose

L1HS-silencing plasmid (CRISPRi gRNA2)

Depositor

Johan Jakobsson

Publication

Adami et al bioRxiv 2025.01.17.633315

Insert

LacZ gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Publication

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-N2cG-WPRE

Plasmid

#218757

Purpose

Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

Depositor

Lindsay Schwarz

Publication

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

N2cG

Use

AAV

Mutation

See Depositor Comments

Promoter

hSyn

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CAG-ConVERGD-eGFP-WPRE

Plasmid

#218749

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Publication

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

CAG

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-WPRE

Plasmid

#218745

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Publication

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-W3SL

Plasmid

#218744

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Publication

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_176

Plasmid

#199108

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_168

Plasmid

#199100

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Publication

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

Plasmid

#216533

Purpose

An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

Depositor

Yuki Kambe

Publication

Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

Insert

axon-jGCaMP8m

Use

AAV and Cre/Lox

Tags

GAP43

Expression

Mammalian

Promoter

hSynapsin1

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Publication

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Tags

myc

Expression

Mammalian

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

Plasmid

#170467

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Publication

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK1214 (pNR-HC)

Plasmid

#252052

Purpose

Empty vector control that does not express double-stranded RNA.

Depositor

Jeffrey Barrick

Publication

Navarro-Escalante et al bioRxiv 2026.01.18.700149

Type

Empty backbone

Use

Control

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pC0.338

Plasmid

#200626

Purpose

Level 0 part - TSS promoter (for assembly with level 0 RBS part)

Depositor

Alistair McCormick

Publication

Astbury et al bioRxiv 2026.02.24.707249

Insert

trc10_TSS-RBS

Use

Synthetic Biology

Available since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pC0.335

Plasmid

#200623

Purpose

Level 0 part - TSS promoter (for assembly with level 0 RBS part)

Depositor

Alistair McCormick

Publication

Astbury et al bioRxiv 2026.02.24.707249

Insert

V02_TSS-RBS

Use

Synthetic Biology

Available since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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