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Plasmid#105114Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-Myc/nYFP-HAExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-CN
Plasmid#105113Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneUseTagscYFP-HA and nYFP-MycExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAttB-Tub-HA-WnkDeltaCC
Plasmid#203972PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domains 1 and 2PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCC1
Plasmid#203973PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domain 1PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCC2
Plasmid#203974PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationdeletion of coiled coil domain 2PromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub-HA-WnkDeltaCT
Plasmid#203975PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expressionDepositorInsertWnk (Wnk Fly)
UseTagsHAExpressionInsectMutationC-terminal deletion after RFxV motifPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS(B)-Rheb-Q64L
Plasmid#21050DepositorInsertRas-homolog enriched in brain (RHEB Human)
UseRetroviralTagsFLAGExpressionBacterialMutationglutamine 64 to leucinePromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV let-7g
Plasmid#14784DepositorInsertlet-7g
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTBRII / CEP-Zeo / Hygro
Plasmid#16622DepositorInsertTGF beta type II receptor (TGFBR2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtnluc
Plasmid#184339PurposeTransformation of mitochondrial DNA in yeastDepositorInsertnanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]
Plasmid#124069PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxTagsExpressionInsectMutationPromoterbhsp68Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL815N
Plasmid#52323PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDifferentiation
Plasmid#228393Purposeintended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithographyDepositorInsertpLambda-luxR+LVAtag__pLux-mRuby2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG-CSF2RB
Plasmid#125750PurposeLentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES eGFPDepositorInsertCSF2RB(wt) (CSF2RB Human, 2694)
UseLentiviralTagsExpressionMutationcodon optimizedPromoterSFFVAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only