We narrowed to 23,671 results for: CRISPR

1. 6xHis-MBP-huTsCas12a

   Plasmid

   #174687

   Purpose

   Expresses 6xHis-MBP tagged TsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huTsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2_8xCTS-ECFP

   Plasmid

   #200249

   Purpose

   Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   8xCTS-ECFP
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-MBP-huBsCas12a

   Plasmid

   #174671

   Purpose

   Expresses 6xHis-MBP tagged BsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScCas9

   Plasmid

   #117700

   Purpose

   Mammalian expression vector encoding WT ScCas9

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

   Insert

   Streptococcus canis Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-mCherry empty

   Plasmid

   #80975

   Purpose

   Cas9, mCherry, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXT130_TwinStrep-SUMO-ShTnsC

   Plasmid

   #135526

   Purpose

   Protein expression construct for ShTnsC

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsC
   

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXT131_TwinStrep-SUMO-ShTniQ

    Plasmid

    #135527

    Purpose

    Protein expression construct for ShTniQ

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTniQ
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXT129_TwinStrep-SUMO-ShTnsB

    Plasmid

    #135525

    Purpose

    Protein expression construct for ShTnsB

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRVV661

    Plasmid

    #108282

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH375

    Plasmid

    #86841

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA3

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNR4A1-donor

    Plasmid

    #112295

    Purpose

    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

    Depositor

    Kevin White

    Insert

    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    

    Use

    CRISPR

    Mutation

    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRVV669

    Plasmid

    #108283

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p426*-SaSgH

    Plasmid

    #136994

    Purpose

    Helper plasmid for SaCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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