We narrowed to 25,364 results for: ina

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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174529

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Publication

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

PylS
PylT

Tags

Flag

Expression

Mammalian

Promoter

U6

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-N-miRFP670-C

Plasmid

#159438

Purpose

CRISPR knock-in donor construct with fluorescent reporter

Depositor

Jianhua Xing

Publication

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Promoter

CMV

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Publication

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Co-Rest/RCOR1 [K72/8R]

Plasmid

#140066

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/8.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Co-Rest/RCOR1 (Human) recombinant mouse monoclonal antibody (RCOR1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GIT2 [N83/48R]

Plasmid

#140079

Purpose

Mammalian Expression Plasmid of anti-GIT2 (Human). Derived from hybridoma N83/48.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GIT2 (Human) recombinant mouse monoclonal antibody (GIT2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-SUR2B [N323B/20R]

Plasmid

#114480

Purpose

Mammalian Expression Plasmid of anti-SUR2B (Rat). Derived from hybridoma N323B/20.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SUR2B (Rattus norvegicus) recombinant mouse monoclonal antibody (Abcc9 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJHG004

Plasmid

#126455

Purpose

Expresses dTdT in mammalian cells.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dTdT (DNTT Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate the enzyme.

Promoter

CMV

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-SAPAP [N127/31.1R]

Plasmid

#114529

Purpose

Mammalian Expression Plasmid of anti-Pan-SAPAP (Rat). Derived from hybridoma N127/31.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-SAPAP (Rattus norvegicus) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-3 [N278/19R]

Plasmid

#114476

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-3 (Mouse). Derived from hybridoma N278/19.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-3 (Mus musculus) recombinant mouse monoclonal antibody (Syt3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_RapACR_T111C_N248Q-EYFP

Plasmid

#119197

Purpose

Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

Depositor

John Spudich

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Anion channelrhodopsin RapACR fast mutant T111C_N248Q

Tags

EYFP

Expression

Mammalian

Mutation

changed threonine 111 to cysteine and asparagine …

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-SUR1 [N289/16R]

Plasmid

#114556

Purpose

Mammalian Expression Plasmid of anti-SUR1 (Rat). Derived from hybridoma N289/16.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SUR1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Abcc8 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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bactin:mKate2-ras

Plasmid

#105963

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Ras (KRAS Human)

Tags

mKate2

Expression

Bacterial

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PBD H538A/K540M

Plasmid

#60879

Purpose

Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

Depositor

Trina Schroer

Publication

Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

Insert

Polo-like kinase 1 (PLK1 Human, BC01486)

Tags

GST

Expression

Bacterial

Mutation

H538A/K540M

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat K193A

Plasmid

#32450

Depositor

Anthony Means

Publication

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

K193A (S3V, G96R, and P108L also present)

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
3604 pGEX4T-1 Bad S136A (104-141)

Plasmid

#8801

Depositor

Stanley Korsmeyer

Publication

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S136A

Promoter

Ptac

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET24a MDH_STRAW

Plasmid

#204240

Purpose

Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_STRAW (mdh Streptomyces avermitilis)

Tags

6X His

Expression

Bacterial

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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G421C_BattB(GA)_mCherry

Plasmid

#200633

Purpose

Strong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad

Depositor

Kenneth Matreyek

Publication

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

mCherry

Expression

Mammalian

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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