We narrowed to 32,985 results for: puromycin

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SRSF2 A9.3 gRNA

Plasmid

#90902

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SRSF2 A10.3 gRNA

Plasmid

#90903

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRSF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SRSF2 (Guide Designation A10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-Rheb-HVR HRas-IP

Plasmid

#13833

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb-HVR HRas (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminal of Rheb and hyper variable region (HVR…

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
p6869 pOZ-N puro HA-HPV18 E2

Plasmid

#35134

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E2 (E2 HPV18)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

LTR

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
LIN52 D5.4 gRNA

Plasmid

#90734

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D5.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R5C B11.5 gRNA

Plasmid

#90853

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5C (Guide Designation B11.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-C/pIRES puro Glue (S778-A1056)

Plasmid

#73456

Purpose

Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-N/pIRES puro Glue (M1-A497)

Plasmid

#73454

Purpose

Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, Ha tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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NUMA1 H11.2 gRNA

Plasmid

#90808

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUMA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUMA1 (Guide Designation H11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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TUBB F2.4 gRNA

Plasmid

#90925

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBB (Guide Designation F2.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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p8141 MSCV-P C-FlagHA 18E7 D10K

Plasmid

#163306

Purpose

Expresses HPV18 E7 D10K

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 D10K

Use

Retroviral

Tags

FlagHA

Mutation

D10K

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Non-targeting CRISPRi plasmid

Plasmid

#154891

Purpose

Human non-targeting CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

Insert

Nontargeting gRNA

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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NUMA1 H12.2 gRNA

Plasmid

#90809

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUMA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NUMA1 (Guide Designation H12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-TdT-guide1-PGK-Puro

Plasmid

#102903

Purpose

EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

TdT-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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