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pET28 basic.3

Plasmid

#191881

Purpose

Encodes a GFP design, basic.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S V68A S72A Y145S L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.2

Plasmid

#191880

Purpose

Encodes a GFP design, mid.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.2

Tags

His Tag

Expression

Bacterial

Mutation

T108I Y145I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.3

Plasmid

#191877

Purpose

Encodes a GFP design, slow.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.3

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S T167V L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.2

Plasmid

#191911

Purpose

Encodes a GFP design, basic.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.2

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145V L220V V224I

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184690

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen (-)NLS

Plasmid

#184698

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184699

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Expression

Plant

Promoter

FRO6

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI046

Plasmid

#170842

Purpose

(pUBQ10::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pUBQ10::SMT2-FLAG::tOCS

Expression

Plant

Promoter

pUBQ10

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1-cloDF-kan

Plasmid

#184836

Purpose

Expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM4_14

Plasmid

#183062

Purpose

Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Inserts

mCherry
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HREsYGP1p and RET2

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJCble

Plasmid

#185861

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

DHFR P66L

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_6xH

Plasmid

#185712

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KBB430

Plasmid

#185091

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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