We narrowed to 15,951 results for: grna

1. pTAG2E

   Plasmid

   #176865

   Purpose

   First sgRNA in a tRNA separated array of sgRNAs

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   sgRNA_2E
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TmiRc3_shURB5

   Plasmid

   #81066

   Purpose

   Gateway entry vector encoding shRNA to UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   shRNA targeting UBR5
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroSuper TIF1gamma

   Plasmid

   #15728

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   TIF1 gamma shRNA (TRIM33 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLKO.1-PRAS40

   Plasmid

   #15477

   Depositor

   Do-Hyung Kim

   Article

   Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

   Insert

   PRAS40 shRNA (AKT1S1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pJEC581

   Plasmid

   #174369

   Purpose

   RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   RFP with PAM rich sequence upstream promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23117

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA2867

   Plasmid

   #25439

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   mCherry-miR155-lacZalpha (Mir155hg Mouse, Discosoma-mouse-E. coli)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330A_D10A-1x6

   Plasmid

   #58776

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-blast shGAC

   Plasmid

   #110410

   Purpose

   shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA Pol III)

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV.PARP1#3

   Plasmid

   #14546

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pMKO.1 GFP B56 alpha shRNA 6-3

    Plasmid

    #10680

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 alpha shRNA (PPP2R5A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pEN_TmiRc3_shNT

    Plasmid

    #81067

    Purpose

    Gateway entry vector encoding non-targeting shRNA

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    non-targeting shRNA
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISP_IS

    Plasmid

    #120425

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    constitutive

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroSuper-shYes

    Plasmid

    #26987

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Yes (YES1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSicoR-mCh-GFPi

    Plasmid

    #21905

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    GFPi
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSuper-Rem2-2

    Plasmid

    #51595

    Purpose

    shRNA 2 against rodent Rem2

    Depositor

    Suzanne Paradis

    Article

    Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

    Insert

    Rem2 (Rem2, Rem2 Rat, Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pRetroSuper-GFP TIF1gamma

    Plasmid

    #15721

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma shRNA (TRIM33 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pLV(shRNA)-Puro-U6-CCEPR

    Plasmid

    #108277

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-hU6-mU6-Cre2GO

    Plasmid

    #136907

    Purpose

    Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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