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pMUT2-pFF-41.5-mCherry

Plasmid

#192863

Purpose

pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Oxygen inducible promoter

Expression

Bacterial

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rScarlet

Plasmid

#205759

Purpose

Expression of rScarlet in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rScarlet

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsTagRFP

Plasmid

#205757

Purpose

Expression of rsTagRFP in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsTagRFP

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Expression

Bacterial

Promoter

Ptac

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFusionRed1

Plasmid

#205758

Purpose

Expression of rsFusionRed1 in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFursionRed1

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mRuby-Sec23A

Plasmid

#36158

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23A (SEC23A Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pTrig-CopA

Plasmid

#104578

Purpose

Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PCopA-RiboJ-B0034-repL

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Human NCKX6 (pcDNA3.1+)

Plasmid

#75216

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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PMT-mKate2

Plasmid

#203785

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mKate2

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB103-Citrine

Plasmid

#79137

Purpose

Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

Depositor

Yutaka Kodama

Article

Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

Insert

Citrine

Tags

Citrine

Expression

Plant

Promoter

MpEF1a promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEB1-Sapphire

Plasmid

#103976

Purpose

Plasmid encoding Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S72A, Y145F,…

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pECT3

Plasmid

#75450

Purpose

Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt3

Expression

Bacterial

Promoter

none

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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