We narrowed to 37,933 results for: Nov
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Plasmid#174120PurposeExpresses ABE8e with an EF1a promoterDepositorInserteABE
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human b4 nicotinic receptor subunit EM construct
Plasmid#163961PurposepEZT-BM vector with beta4 gene after CMV promoter, published EM constructDepositorInsertHuman beta4 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…TagsStrepII tag and apocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationPro341-Ser395 replaced with apocytochrome b(562)R…PromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCIDR
Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-Spastin(87-616)DeltaExon4
Plasmid#128794PurposeBacteiral expression of Spastin M87 varient without exon 4DepositorInsertSpastin (SPAST Human)
Use`TagsGSTExpressionBacterialMutationresidues 87-616 without exon 4.PromoterT7Available sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN410 (FuGW-S(E2F1)p-ECFP sponge control)
Plasmid#105182PurposeModule 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)DepositorInsertS(E2F1)p-ECFP (Cerulean) sponge control
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-CFP-FRBx5
Plasmid#103780Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-CFP-FRBx5
ExpressionMammalianPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
CIAR_pcDNA5/FRT/TO
Plasmid#86502PurposeExpresses CIAR in mammalian cells. Used to generate Flp-In stables.DepositorInsertCIAR (SOS1 Human)
UseFrtExpressionMammalianMutationT968L in SOScatPromoterCMV (tet operator)Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pconst #5-RFP
Plasmid#81079Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23110Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MKRN1 WT
Plasmid#78766PurposeTo detect localization of MKRN1 in live mammalian cellsDepositorAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-Dopa5
Plasmid#63854PurposeThis pFTV1 vector contains a dopamine binding riboswitch, controlling the translation initaition rate of luciferase gene.DepositorInsertDopa5 dopamine riboswitch
UseSynthetic BiologyPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
Tags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
(GGS)9_(+36GFP)-Cre.ape
Plasmid#71747Purpose+36-GFP-Cre fusion expression in E.ColiDepositorInsert(GGS)9_+36GFP-Cre
Tags6XHisExpressionBacterialAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NKP33 GFP (1032)
Plasmid#62022Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT7 LDIG69AAAA
Plasmid#61560PurposeHs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutantDepositorAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV Sbf-Sal linker
Plasmid#50434PurposeCloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).DepositorPublicationTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by