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pEBTet-SNAP-CP110

Plasmid

#136802

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPN441

Plasmid

#137873

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:AVRa1-V1

Plasmid

#132357

Purpose

Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

AVRa1-V1 ∆SP with stop codon

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3B G120

Plasmid

#123094

Purpose

Expresses 3xFLAG-LC3B G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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KAT14-pDEST10

Plasmid

#118382

Purpose

HIS-tagged insect cell expression vector with KAT14.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Tags

HIS

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-XPO1-E571K

Plasmid

#116708

Purpose

Lentiviral expression of XPO1 E571K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

XPO1 (XPO1 Human)

Use

Lentiviral

Mutation

E571K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-C420R

Plasmid

#116457

Purpose

Lentiviral expression of PIK3CA C420R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

C420R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAPK1-E322K

Plasmid

#116432

Purpose

Lentiviral expression of MAPK1 E322K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Mutation

E322K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-S310F

Plasmid

#116349

Purpose

Lentiviral expression of ERBB2 S310F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

S310F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R1275Q

Plasmid

#116111

Purpose

Lentiviral expression of ALK R1275Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R1275Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP6

Plasmid

#80662

Purpose

Encodes the synthetic coiled coil peptide SYNZIP6

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP6

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP5

Plasmid

#80661

Purpose

Encodes the synthetic coiled coil peptide SYNZIP5

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP5

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307 hcRed1

Plasmid

#117732

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1807_pmax-dCasRx-FKBP

Plasmid

#118640

Purpose

Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-FKBP

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1803_pmax-RBM38-dCasRx

Plasmid

#118637

Purpose

Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_ECFP

Plasmid

#118857

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

ECFP

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG R367K

Plasmid

#118626

Purpose

Overexpresses ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG ( R367K DNA Binding mutant) (ERG Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

R367K

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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