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pAS_4xMx PylT_FLAG-Mx PylRS

Plasmid

#140011

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST-CMV 3xFLAG-LC3C-GFP

Plasmid

#123110

Purpose

Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabe JunD-HA neo

Plasmid

#58486

Purpose

mammalian expression of mouse JunD

Depositor

Kevin Janes

Publication

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Tags

HA

Expression

Mammalian

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK MEK5-DD-mRFP1 neo

Plasmid

#47548

Purpose

Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Publication

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD-mRFP1 (MAP2K5 Human)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Mutation

S311D, T315D

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

Plasmid

#18017

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V ECFP-W67G

Tags

ECFP-W67G and Venus

Expression

Mammalian

Mutation

mglB-D154A; non-functional ECFP

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pMpGWB115

Plasmid

#68569

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Publication

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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ML6

Bacterial strain

#61908

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE, avtA genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Publication

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial resistance

Kanamycin

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
Dendra1-N1

Plasmid

#54624

Purpose

Localization: N1 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Type

Empty backbone

Tags

Dendra1

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mGeos-M-C1

Plasmid

#54815

Purpose

Localization: C1 Cloning Vector, Excitation: 503, Emission: 514, reversibly switches on/off with UV. (Originally reported as Excitation: 503 / 569, Emission: 514 / 581)

Depositor

Michael Davidson

Type

Empty backbone

Tags

mGeos-M

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
iLOV-C1

Plasmid

#54591

Purpose

Localization: C1 Cloning Vector, Excitation: 450, Emission: 495

Depositor

Michael Davidson

Type

Empty backbone

Tags

iLOV

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3/BLM

Plasmid

#111766

Purpose

To express human BLM protein in human cells

Depositor

Ian Hickson

Publication

Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

Insert

BLM (BLM Human)

Tags

Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

Expression

Mammalian

Promoter

CMV

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE-Tau-cYFP

Plasmid

#92205

Purpose

Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Publication

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(156-239)

Expression

Mammalian

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pBABE-Tau-nYFP

Plasmid

#92204

Purpose

Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Publication

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Publication

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK Tgfbr3-HA neo

Plasmid

#58508

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Publication

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Publication

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1 Myc-huMEKK3 (K391A)

Plasmid

#44157

Depositor

Xin Lin

Publication

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

MEKK3 (K391A) (MAP3K3 Human)

Tags

Myc

Expression

Mammalian

Mutation

K391A, kinase-dead mutant

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 5'cMyc-SMCl- S957A/S966A

Plasmid

#32372

Depositor

Michael Kastan

Publication

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S957A/S996A

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article