-
Plasmid#210262PurposeInducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationsiRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mEGFP-Gphn_P1
Plasmid#194978PurposeAAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoterDepositorInsertmEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
UseAAVTagsmEGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD437
Plasmid#168463PurposeFor the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD438
Plasmid#168464PurposeFor the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD440
Plasmid#168466PurposeFor the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mCherry-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD441
Plasmid#168467PurposeFor the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEGFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD442
Plasmid#168468PurposeFor the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-eYFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD444
Plasmid#168470PurposeFor the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mNeonGreen-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
xCIAR_pcDNA5/FRT/TO
Plasmid#86504PurposeExpresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.DepositorInsertxCIAR (SOS1 Human)
UseFrtTagsExpressionMammalianMutationF929A and T968L in SOScatPromoterCMV (tet operator)Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
P2_DD-HNF4A8_C106R
Plasmid#31096DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsFKBP L106P and mycExpressionMammalianMutationsplice variant 8 derived from promoter P2 with zi…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas ServiceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP
Plasmid#55641PurposeFlp-Dependent EYFPDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-RPB1-His WT CRres si2,4R
Plasmid#139404PurposeDox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorInsertRPB1 (POLR2A Human)
UseTags6xHisExpressionMammalianMutationPromoterpCMV, TRexAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon DREADD Gi-mCherry
Plasmid#177669PurposeExpresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cellsDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2
Plasmid#183352PurposeFlp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)DepositorInsertTVA, EGFP and tTA
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)
Plasmid#55614PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cellsDepositorInsert3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationC4762 mutated to serine to render HERC2 catalytic…PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-RPB1-His K1268R CRres si2,4R
Plasmid#139405PurposeDox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.DepositorInsertRPB1 (POLR2A Human)
UseTags6xHisExpressionMammalianMutationK1268RPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only