Addgene

We narrowed to 17,046 results for: URE

Showing: 1041 - 1060 of 17046 results

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Use

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F04

Plasmid

#71030

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sin3A (Sin3A Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B10

Plasmid

#70840

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sce (Sce Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C08

Plasmid

#70911

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf5 (Taf5 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2C05

Plasmid

#70891

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc2 (Orc2 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha N90F

Plasmid

#58257

Purpose

bacterial expression of human PITP alpha N90F

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

N90F (abolishes PI binding)

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha K61A

Plasmid

#58258

Purpose

bacterial expression of human PITP alpha K61A

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
MSP712

Plasmid

#65768

Purpose

Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Mutation

D10A and H840A mutations in Cas9

Promoter

T7 (x2)

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS3

Plasmid

#128107

Purpose

Express HA tagged Nrxn1α-LNS3

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS3 (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS1

Plasmid

#128105

Purpose

Express HA tagged Nrxn1α-LNS1

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS1 (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD

Plasmid

#98669

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pALB-GFP

Plasmid

#55759

Purpose

Fluorescent marker for hepatocytic lineage

Depositor

Snorri Thorgeirsson

Article

Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Insert

albumin enhancer/promoter (Alb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Mouse Albumin Enhancer/Promoter

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pC034 - LwCas13a-msfGFP-2A-Blast

Plasmid

#91924

Purpose

Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[30Q]

Plasmid

#33236

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 29 amino acids (27 Q…

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA1 Flag ATXN1[85Q]

Plasmid

#33237

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 85 Glutamines and no…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[2Q]

Plasmid

#33235

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q331K

Plasmid

#98673

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Q331K

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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