We narrowed to 2,567 results for: gem

1. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Robert Langer, Vikash Chauhan, Phil Sharp

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet-1:ChrA

   Plasmid

   #225079

   Purpose

   Expresses precursor peptide ChrA in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-J23100

   Plasmid

   #72963

   Purpose

   Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23100 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-SEP_NR2B

   Plasmid

   #23998

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   NR2B (Grin2b Rat)
   

   Tags

   NR2B signal sequence (aa1-31) and SEP (supereclip…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-SEP_NR2A

   Plasmid

   #23997

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   NR2A (Grin2a Rat)
   

   Tags

   SEP

   Expression

   Mammalian

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Akt2

   Plasmid

   #86593

   Purpose

   Expresses eGFP-tagged human Akt2(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2 (AKT2 Human)
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Adeno Cas9

   Plasmid

   #64072

   Purpose

   Adenoviral vector for the expression of the Cas9

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   FLAG

   Promoter

   U6 and CBh

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-Akt2(WT)

    Plasmid

    #86623

    Purpose

    Expresses mCherry-tagged human Akt2(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2 (AKT2 Human)
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Stx1a-12aa-mCer

    Plasmid

    #53236

    Purpose

    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    Syntaxin 1A (Stx1a Rat)
    

    Tags

    12aa-linker and mCerulean

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Fuw CBX8

    Plasmid

    #121111

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CaMKIIa-C1C2-EYFP

    Plasmid

    #35519

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p006-GFP-Logic-AND

    Plasmid

    #112237

    Purpose

    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Inserts

    GFP under control of doubly-repressible promoter
    GFP
    araC
    

    Expression

    Bacterial

    Promoter

    AND-gate and pLacI

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas2C

    Plasmid

    #82374

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBPhsFlp2::PEST

    Plasmid

    #63171

    Purpose

    Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    hsFlp2::PEST
    

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV-EF1aGFP-W

    Plasmid

    #159291

    Purpose

    Lentiviral vector expressing GFP driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human EF1a promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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