We narrowed to 2,503 results for: gem

1. pCas34

   Plasmid

   #82386

   Purpose

   sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBPhsFlp2::PEST

   Plasmid

   #63171

   Purpose

   Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   hsFlp2::PEST
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBP-J23100

   Plasmid

   #72963

   Purpose

   Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23100 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV-EF1aGFP-W

   Plasmid

   #159291

   Purpose

   Lentiviral vector expressing GFP driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CaMKIIa-C1C2-TS-EYFP

   Plasmid

   #35520

   Depositor

   Karl Deisseroth

   Article

   Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

   Insert

   ChR1-ChR2 Chimera
   

   Use

   Lentiviral

   Tags

   Trafficking signal and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Vikash Chauhan, Phil Sharp, Robert Langer

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUB-Cas9-@GL1

   Plasmid

   #86779

   Purpose

   Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   sgRNA against GLABROUS1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis U6-26 promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLG1-puro Non-targeting sgRNA 1

   Plasmid

   #109002

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-puro-mouse Oct4

    Plasmid

    #21110

    Depositor

    Dean Tantin

    Article

    Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRF2-HA

    Plasmid

    #200209

    Purpose

    Expression of full length mTRF2

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TRF2-HA (Terf2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-basic-Polr2a promoter_600bps

    Plasmid

    #21111

    Depositor

    Dean Tantin

    Article

    Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

    Insert

    Polr2a promoter_600bps (POLR2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pASH3 [pmyo-3::BeCNG1-YFP]

    Plasmid

    #168167

    Purpose

    Expression of BeCNG1-YFP in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    BeCNG1-YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFP-Ub-VPS13D_dN

    Plasmid

    #176465

    Purpose

    express VPS13D lacking N-terminal 117 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas2D

    Plasmid

    #82375

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid

    #225442

    Purpose

    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

    Depositor

    Diego Orzaez

    Insert

    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a:ChrI

    Plasmid

    #225085

    Purpose

    Expresses anthrone-oxygenase like enzyme ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrI
    

    Use

    Tags

    His6 and His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge