We narrowed to 90,070 results for: Mal

1. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

   Plasmid

   #160046

   Purpose

   For expressing the full paradoxical circuit

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Inserts

   osTIR1
   iaaH
   iCasp9
   BlastR
   

   Use

   Synthetic Biology

   Tags

   mAID, mCherry, and mGFPmut3

   Expression

   Mammalian

   Promoter

   no promoter, T2A is used and pEF1s

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIG AKT3 E17K

   Plasmid

   #73050

   Purpose

   Human AKT3 E17K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K (AKT3 Human)
   

   Expression

   Mammalian

   Mutation

   E17K

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG5-P190

   Plasmid

   #31285

   Depositor

   Nora Heisterkamp

   Article

   Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

   Insert

   Bcr-abl fusion protein P190 (ABL1, BCR Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac-FLAG-mProser1-IRES-Blast

   Plasmid

   #226172

   Purpose

   Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

   Insert

   Proline Serine Rich protein 1 (Proser1 Mouse)
   

   Tags

   2X FLAG

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-TRE-FH-TET3 short

   Plasmid

   #166916

   Purpose

   Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   TET3 short isoform
   

   Use

   Lentiviral

   Tags

   HA Tag

   Expression

   Mammalian

   Mutation

   Changed nucleotide 18 (G) to (A) to delete FseI r…

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV302

   Plasmid

   #119943

   Purpose

   Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

   Insert

   N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICOZ-SB100X[EF1α-CD19 4-1BB]

    Plasmid

    #218328

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

    Plasmid

    #161577

    Purpose

    Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Expression

    Mammalian

    Promoter

    EF1a driving selection cassette

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBa-flag-BicD2 594-FKBP

    Plasmid

    #64206

    Purpose

    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Bicaudal D protein (Bicd2 Mouse)
    

    Tags

    FKBP and flag

    Expression

    Mammalian

    Mutation

    no start methionine, aa1-594

    Promoter

    chicken Beta Actin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG mFoxg1-GFP wildtype

    Plasmid

    #73048

    Purpose

    C-terminal fusion of gfp Mouse wildtype Foxg1

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    mFoxg1 (Foxg1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-RR-ABE8e-V106W

    Plasmid

    #193656

    Purpose

    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM60-FLAG-SLC38A9.1 (delta110)

    Plasmid

    #71861

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, missing first 110 amino acids

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQLL-Tc1-1_Xt[EF1α-copGFP]

    Plasmid

    #218325

    Purpose

    Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRES2-EGFP-SHP2DA

    Plasmid

    #12286

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2DA (PTPN11 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Aspartic Acid 425 mutated to Alanine

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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