We narrowed to 23,671 results for: CRISPR

1. pCAG-SpCas9-P2A-EGFP (MSP2582)

   Plasmid

   #223067

   Purpose

   pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

   Plasmid

   #113159

   Purpose

   An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HK1 gRNA (BRDN0001146994)

   Plasmid

   #77645

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human HK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   HK1 (HK1, HOOK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-dCas9-p300 Core (C1204R)

   Plasmid

   #61361

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVTR(T7A)-Cas9-T2A-mClover3

   Plasmid

   #172293

   Purpose

   In vitro transcription of Cas9-T2A-mClover3 synthetic construct

   Depositor

   Patrik Verstreken

   Insert

   Cas9-T2A-mClover3
   

   Use

   CRISPR

   Promoter

   T7 promoter A-initiating

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-tetO-EF1a-GFP

   Plasmid

   #187645

   Purpose

   Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites

   Depositor

   Martin Carroll

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY125 (pcDNA3.1-huBfCpf1)

   Plasmid

   #92301

   Purpose

   Expression of humanized BfCpf1

   Depositor

   Feng Zhang

   Insert

   huBfCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY115 (pcDNA3.1-huMlCpf1)

   Plasmid

   #92275

   Purpose

   Expression of humanized MlCpf1

   Depositor

   Feng Zhang

   Insert

   huMlCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY112 (pcDNA3.1-huSaCpf1)

   Plasmid

   #92269

   Purpose

   Expression of humanized SaCpf1

   Depositor

   Feng Zhang

   Insert

   huSaCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTJK644

    Plasmid

    #138012

    Purpose

    SIX1 knockout

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA2 (SIX1 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    None

    Promoter

    U6

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1162BY-CMV-mCherry-bGHpolyA

    Plasmid

    #235172

    Purpose

    Expresses mCherry

    Depositor

    Omar Akbari

    Insert

    mCherry
    

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1162BX-CMV-EGFP-bGHpolyA

    Plasmid

    #235171

    Purpose

    Expresses EGFP

    Depositor

    Omar Akbari

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNM220_AAVS1 integration helper

    Plasmid

    #211864

    Purpose

    Cas9 cutting at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    AAVS1
    

    Use

    CRISPR

    Mutation

    N/A

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEL15_MATz

    Plasmid

    #166099

    Purpose

    Expression of gRNA targeting the MAT locus

    Depositor

    Rebecca Burgess

    Insert

    MAT Z1
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTJK635

    Plasmid

    #138009

    Purpose

    SIX1 knockout

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA2 (SIX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTJK643

    Plasmid

    #138011

    Purpose

    SIX1 knockout

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA1 (SIX1 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    None

    Promoter

    U6

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTJK642

    Plasmid

    #138013

    Purpose

    SIX1 CRISPRi

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA13 (SIX1 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    None

    Promoter

    U6

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid

    #131312

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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