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pLXSN-H-Ras_V12-E38

Plasmid

#39520

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 E38 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and D38E

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-YA/VD-Myc

Plasmid

#60701

Purpose

mammalian expression of Myc tagged rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-S35

Plasmid

#39505

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Expression

Mammalian

Mutation

G12V and T35S

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-G37

Plasmid

#39506

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Expression

Mammalian

Mutation

G12V and E37G

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-C40

Plasmid

#39508

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Expression

Mammalian

Mutation

G12V and Y40C

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-G37

Plasmid

#39518

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and E37G

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-C40

Plasmid

#39521

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a W118S

Plasmid

#40042

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Tryptophane 118 to Serine

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a V21A

Plasmid

#40041

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Valine 21 to Alanine

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-A38

Plasmid

#39519

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 A38 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and D38A

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pN1_HsKIF1C(1-376)-NC-LZ-TagBFP-FRB

Plasmid

#253326

Purpose

mammalian expression of the motor domain of human KIF1C tagged with TagBFP and FRB. NC and LZ facilitate stable dimerization. FRB enables rapamycin-induced attachment to FKBP-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

TagBFP, FRB

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-KLF17-CRa1-MS2

Plasmid

#251117

Purpose

sgRNA1 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA1 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-KLF17-CRa2-MS2

Plasmid

#251118

Purpose

sgRNA2 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+ Flag-SRSF3_WobbleMut

Plasmid

#237458

Purpose

Vector for constitutive mammalian expression of Flag-tagged full-length SRSF3 mutated to be resistant to SRSF3 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

SRSF3

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pET01-NRF1-Ex7-minigene

Plasmid

#237459

Purpose

Vector for constitutive expression of NRF1 alternative exon-7 minigene

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7 minigene

Expression

Mammalian

Promoter

RSV LTR

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLCHKOv4

Plasmid

#237451

Purpose

Lentiviral backbone for expressing U6-driven hybrid guide (hg)RNAs for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLCHKOv5

Plasmid

#237452

Purpose

Lentiviral backbone for expressing doxycycline (Dox)-inducible, Pol II-driven hybrid guide (hg)RNAs.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-fibrillarin-FRT

Plasmid

#247341

Purpose

FLP/FRT based mammalian expression vector for EGFP-fibrillarin driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Imai et al Mol Biol Cell. 2017 Nov 7;28(23):3349-33

Insert

fibrillarin (FBL) (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCW-MRTFA-HA

Plasmid

#247436

Purpose

siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

MRTF-A (Mrtfa Mouse)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Mutation

siRNA resistant - no amino acid mutations

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCW-Rho(G14V)-HA

Plasmid

#247437

Purpose

HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.

Depositor

Richard Treisman

Article

Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:

Inserts

RhoA(G14V) (RHOA Human)
IRES2:EGFP

Use

Lentiviral; Dox-inducible

Tags

HA

Expression

Mammalian

Mutation

G14V mutation (constitutive active mutant)

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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