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pTol1-UAS:ChR2(H134R)-tdTomato

Plasmid

#124237

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChR2(H134R)-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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TPST1-EGFP

Plasmid

#66617

Purpose

Encodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Article

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST1 (TPST1 Human)

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pDGE497

Plasmid

#153249

Purpose

M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS R4-R3

Plasmid

#62086

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAGT9050

Plasmid

#219429

Purpose

PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

AATG_PapE-4xLF2_AGGT

Use

Moclo compatible level 0 module

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-MSK1

Plasmid

#165601

Purpose

Expresses Sp dCas9 fused to human MSK1

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

ribosomal protein S6 kinase A5 (RPS6KA5 Human, S. Pyogenes, Synthetic)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSF-1Rwt-IRES-GFP

Plasmid

#115523

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

human CSF-1 receptor (CSF1R Human)

Use

Retroviral

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2a

Plasmid

#186818

Purpose

Fluorescent reporter for Camk2a expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2a promoter (Camk2a Mouse)

Use

Luciferase

Promoter

Camk2a promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2b

Plasmid

#186819

Purpose

Fluorescent reporter for Camk2b expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2b promoter (Camk2b Mouse)

Use

Luciferase

Promoter

Camk2b promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGl3-Camk2g

Plasmid

#186821

Purpose

Fluorescent reporter for Camk2g expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2g promoter (Camk2g Mouse)

Use

Luciferase

Promoter

Camk2g promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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MTDH-His-bio

Plasmid

#52331

Purpose

Expresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

MTDH (MTDH Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Circular-ic-CVB3-GFP

Plasmid

#226260

Purpose

Production of in situ circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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M_NLS 41kDa

Plasmid

#201341

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry2-L

Plasmid

#104003

Purpose

Plasmid encoding mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Circular-ic-EMCV-GFP

Plasmid

#226259

Purpose

Production of in situ circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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HSC1-HS4-GiP

Plasmid

#58540

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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