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pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE NLS

Plasmid

#82466

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt3

Plasmid

#66835

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_RANBP2_Ran-BP2

Plasmid

#109839

Purpose

Protein expression and purification of RANBP2_Ran-BP2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RANBP2_Ran-BP2 (RANBP2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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114_UBI_iCre_crystGFP

Plasmid

#171797

Purpose

Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised iCre under the control of the Ubiquitin promoter

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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10XSTAT92E–luciferase

Plasmid

#37393

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

SOCS36E enhancer fragment (Socs36E Fly)

Use

Luciferase; Jak/stat reporter construct

Expression

Insect

Promoter

Hsp70

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-DARPinOFF7

Plasmid

#182398

Purpose

Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-DARPinOFF7

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H AC editing reporter

Plasmid

#198883

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGA)

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEY41

Plasmid

#191045

Purpose

dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dat-1 (dat-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd2

Plasmid

#42254

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6, LgBiT

Expression

Bacterial

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin1

Plasmid

#41911

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL1 (CUL1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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p3xFlag-CMV7-Erk2

Plasmid

#39223

Depositor

Melanie Cobb

Article

Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

Insert

Erk2 (Mapk1 Rat)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pZS*11_4enh

Plasmid

#149423

Purpose

Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Inserts

rbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer

Tags

T120>A

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pICH47822_HA-Sr62TK

Plasmid

#233535

Purpose

Expresses HA-Sr62TK in planta

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62TK

Tags

HA tag

Expression

Plant

Promoter

35S

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47822_Sr62NLRa

Plasmid

#233537

Purpose

Expresses Sr62NLRa in planta

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62NLRa

Expression

Plant

Promoter

35S

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMW357

Plasmid

#238204

Purpose

Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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