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pTwist-SARS-CoV-2 Spike-deltaC18 D215G (GGT)

Plasmid

#212345

Purpose

Encodes SARS-CoV-2 spike D215G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D215G (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D215G

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L242H (CAT)

Plasmid

#212346

Purpose

Encodes SARS-CoV-2 spike L242H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L242H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L242H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N439K (AAA)

Plasmid

#212332

Purpose

Encodes SARS-CoV-2 spike N439K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N439K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N439K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC)

Plasmid

#212333

Purpose

Encodes SARS-CoV-2 spike S477N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 D936Y (TAT)

Plasmid

#212334

Purpose

Encodes SARS-CoV-2 spike D936Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D936Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D936Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L5F (TTC)

Plasmid

#212335

Purpose

Encodes SARS-CoV-2 spike L5F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaH69V70

Plasmid

#212336

Purpose

Encodes SARS-CoV-2 spike deltaH69, deltaH70 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike deltaH69, deltaH70 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

deltaH69, deltaH70

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 deltaY145

Plasmid

#212337

Purpose

Encodes SARS-CoV-2 spike Y145H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y145H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y145H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N501Y (TAT)

Plasmid

#212338

Purpose

Encodes SARS-CoV-2 spike N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 A222V (GTG)

Plasmid

#212329

Purpose

Encodes SARS-CoV-2 spike A222V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike A222V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A222V

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM2_mut-cGFP

Plasmid

#220213

Purpose

Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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Ypq2-2GFP-URA

Plasmid

#207010

Purpose

Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

YPQ2

Tags

2xGFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Vph1-2GFP-URA

Plasmid

#207009

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

VPH1

Tags

2xGFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN

Plasmid

#209786

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN

Use

AAV

Promoter

TNT4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pI pm

Plasmid

#218098

Purpose

Phagemid that expresses LacI, and pI under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pI

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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LacI pm

Plasmid

#218097

Purpose

Phagemid that expresses LacI but no phage proteins, used as a control

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge