We narrowed to 50,969 results for: LIS
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Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
LcV2-Hygro
Plasmid#91977PurposeLentiCRISPR v2 with Hygro cloned in place of PuroDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PPP2R2B
Plasmid#16181DepositorInsertPP2A regulatory subunit B, beta isoform (PPP2R2B Human)
ExpressionMammalianAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS
Plasmid#132404Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R-6His
Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
TagsHisExpressionBacterialPromoterT7/Sp6Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIFV
Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-CasRX-P2A-EGFP-BGH PolyA
Plasmid#154004PurposeExpress CasRxDepositorInsertCasRx
UseCRISPRPromoterCAGAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
Tagsdimerization dependent fluorescent protein monome…ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-MCU-Flag
Plasmid#50054PurposeExpression of human MCU in mammalian cellsDepositorAvailable SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔRING-Box
Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
TagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
TagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYS1Ptac
Plasmid#85545PurposeConstitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoterDepositorInsertsCpf1
recT
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialPromotertac and unknownAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-ER-TurboID
Plasmid#149412PurposeMuscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5PromotertMCKAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo PFKP-GFP
Plasmid#116940PurposeExpression of PFKP-GFP fusion proteinDepositorAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^Halo
Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioD
Plasmid#65350Purposesysthesis of vioD gene in the violacein pathwayDepositorInsertvioD
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only