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lentiSAMv2 TCF7L2 TSS-guide2

Plasmid

#118185

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 rs11257655-guide2

Plasmid

#118205

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide1

Plasmid

#118196

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 BFP-guide1

Plasmid

#118165

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the BFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 OPTN TSS-guide3

Plasmid

#118198

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 OPTN TSS-guide2

Plasmid

#118197

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 CAMK1D TSS-guide4

Plasmid

#118195

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs11257655-guide4

Plasmid

#118207

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs11257655-guide3

Plasmid

#118206

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 CAMK1D TSS-guide3

Plasmid

#118194

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 CAMK1D TSS-guide1

Plasmid

#118192

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs7903146-guide3

Plasmid

#118204

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 EGFP-guide3

Plasmid

#118168

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 CAMK1D TSS-guide2

Plasmid

#118193

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TB204

Bacterial Strain

#230033

Purpose

Fluorescently labelled (monomeric sfGFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available Since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
TB201

Bacterial Strain

#230031

Purpose

Fluorescently labelled (YFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △trpR

Bacterial Strain

#229546

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, tryptophan overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △hisL

Bacterial Strain

#229552

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, histidine overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
SJ358

Bacterial Strain

#67755

Purpose

a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

Depositor

Suckjoon Jun

Article

Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

Bacterial Resistance

None

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only