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pXD80EN15-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225327

Purpose

Expresses Eggerthella lenta A2 Dadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDR-pBAD-ATG-6xHIS-AsiA

Plasmid

#245835

Purpose

Express anti-sigma protein, AsiA, in E. coli. His-Tag for Western Blots or Purification.

Depositor

Jie Xiao

Article

Weng et al Proc Natl Acad Sci U S A. 2019 Oct 1;116

Insert

AsiA

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mCherry_TEV_mVenus_FG

Plasmid

#240674

Purpose

Expression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_TEV_mVenus_FR

Plasmid

#240663

Purpose

Expression plasmid for mCherry-TEV-mVenus-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mCherry_TEV_mVenus_FR

Plasmid

#240664

Purpose

Expression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-28

Plasmid

#246311

Purpose

Evaluation of PtU6.1c1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

PtU6.1c1 promoter

Use

CRISPR

Expression

Plant

Mutation

C-to-A (-42 from TSS), A-to-C (-41)

Promoter

PtU6.1c1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pKO024.306-107

Plasmid

#242927

Purpose

Expression of a Sth1-dCas9 compatible MS2 scRNA J306 and a Spy-dCas9 compatible sgRNA J107

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

sgRNA J107

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-NHis6NocM-emptyMCS2

Plasmid

#240399

Purpose

expresses N-His tagged NocM (acyl carrier protein) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocM

Tags

His

Expression

Bacterial

Mutation

M1 in native sequence not included in insert

Promoter

T7

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD183_gCAD_pET

Plasmid

#239119

Purpose

Gorilla ACOD1 in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

StrepTag-II and TEV site

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Scramble_gRNA_puro

Plasmid

#234999

Purpose

scramble sgRNA

Depositor

Samrat Roy Choudhury

Article

Choudhury et al Genes Dis. 2025 Sep 6;13(1):101843. doi:

Insert

scramble sgRNA

Use

Lentiviral

Tags

EGFP:T2A:Puro

Expression

Mammalian

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

Plasmid

#233067

Purpose

Piggybac CRISPRi construct

Depositor

Markus Landthaler

Article

Vučićević et al bioRxiv 2024.09.12.612198

Insert

KRAB-dCas9

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BRCA1

Plasmid

#222530

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BRCA1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB1

Plasmid

#222534

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB1 (HMGB1 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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