We narrowed to 12,660 results for: cel.2
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Plasmid#19714DepositorExpressionMammalianMutationmouse Brn2 DNA binding domain-Drosophila engraile…Available SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only
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pAC147-pCR8-dCas9VP64
Plasmid#48219PurposedCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP64MutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
Plasmid#79259PurposeExpresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cellsDepositorInsertDmeIF4G (eIF4G1 Fly)
UseSchneider 2 cell expressionTagsV5-His6 and lambdaN-HAPromoterAc5 promoterAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PLAA-Strep-HA
Plasmid#113484PurposeExpression of human PLAA with C-terminal strep-HA tagDepositorInsertPLAA (PLAA Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
Plasmid#176396PurposeExpresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.DepositorInsertGAL1 promoter, SZ1-RGG-GFP-RGG-RGG
ExpressionYeastPromoterGAL1Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184738Purposeencodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Kif1A(1-489)-DHFR-myc
Plasmid#117833PurposeConstitutively active Kinesin-3 motor tagged with DHFR and mycDepositorInsertKif1A (1-489) (Kif1a Mouse)
TagsDHFR and mycMutationonly aa 1-489 (motor domain)PromoterCMVAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184739Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_CDK7
Plasmid#111684PurposeMAC-tagged gene expressionDepositorInsertCDK7 (CDK7 Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184740Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC148-pCR8-dCas9VP96
Plasmid#48220PurposedCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP96MutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop
Plasmid#240256PurposeAttenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.DepositorInsertnanobody Nb139
TagsNLS-VP64ExpressionMammalianMutationVP64 = 4*VP16[aa437-447]PromoterSFV subgenomic promoterAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
PX458-SPNS1-sg2
Plasmid#198567PurposeSPNS1-targeting sgRNA in PX458 vectorDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA12
Plasmid#196106PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108DepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFB2gRNA13
Plasmid#196107PurposeContains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108DepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E
Plasmid#146204PurposeInsect Expression of DmMe31B-Q281AH284ADepositorInsertDmMe31B-Q281AH284A (me31B Fly)
ExpressionInsectMutationone silent mutation A1101G and two non silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E
Plasmid#146205PurposeInsect Expression of DmMe31B-T288AK292ADepositorInsertDmMe31B-T288AK292A (me31B Fly)
ExpressionInsectMutationone silent mutation A1101G and two non silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p21StBD
Plasmid#190269PurposeConstitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-BDPromoterTDH3Available SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt
Plasmid#175564PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2DepositorInsertGOXL2
TagsCitrineExpressionPlantPromoterRUBYAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only