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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDest2-Amp

Plasmid

#157650

Purpose

Golden Gate assembly of a pET28-compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDest3-Amp

Plasmid

#157651

Purpose

Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CSIV-DEST

Plasmid

#141053

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC

Plasmid

#116988

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC

Plasmid

#116992

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0366_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2_RC

Plasmid

#116978

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0363_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1

Plasmid

#116975

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

Plasmid

#116958

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0365_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2

Plasmid

#116977

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0364_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1_RC

Plasmid

#116976

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

Plasmid

#116957

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0377_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2

Plasmid

#116989

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC

Plasmid

#116990

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1

Plasmid

#116987

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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SYNJ1-145_pCSdest

Plasmid

#53810

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-145 (SYNJ1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CMYA5/pDEST15

Plasmid

#98106

Purpose

Express CMYA5 (aa 4039 – 4069) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5T2/pDEST17

Plasmid

#98118

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Tags

6X-His

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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