We narrowed to 3,216 results for: ER;

1. pGEX Syt1 C2AB (96-421) D365E V304C

   Plasmid

   #170651

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D365E, C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Syt1 C2AB (96-421) I367T F234C

   Plasmid

   #170653

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   I367T, C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Syt1 C2AB (96-421) I367T V304C

   Plasmid

   #170654

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   I367T, C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF(UG) hSyn SYP-deltaTMD SYT7alpha

   Plasmid

   #179718

   Purpose

   Lentiviral expression of synaptic vesicle-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

   Plasmid

   #179722

   Purpose

   Lentiviral expression of lysosome-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pF(UG) U6-SYT7 sgRNA 777 HaloTag

   Plasmid

   #179724

   Purpose

   U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   sgRNA and HaloTag
   

   Use

   Lentiviral

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF(UG) CamKII sf iGluSnFR S72A

   Plasmid

   #179726

   Purpose

   Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   iGluSnFR
   

   Use

   Lentiviral

   Mutation

   S72A

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTorPE-Y-GECO2s

   Plasmid

   #105067

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTorPE-Y-GECO2.1s

   Plasmid

   #105068

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2.1s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSW Syt1-C2B R398,399Q IRES GFP

    Plasmid

    #133819

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    R398,399Q

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW Syt1-C2B K326,327E IRES GFP

    Plasmid

    #133820

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    K326,327E

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179721

    Purpose

    Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Expression

    Mammalian

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rtn4a-mNeonGreen

    Plasmid

    #169032

    Purpose

    Express human Rtn4a

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn4a (RTN4 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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