We narrowed to 32,487 results for: Eng

1. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189922

   Purpose

   AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e
   

   Use

   AAV

   Mutation

   SaCas9 D10A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cbh_v5 AAV-ABE N-terminal

   Plasmid

   #137177

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-ABE N-terminal
   

   Use

   AAV

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC010.AAV

   Plasmid

   #171175

   Purpose

   Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   BBa_J23119 and Sp.pCas9, BBa_J23107

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWPSG-Po-nCas9-BE

   Plasmid

   #195740

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Po (phoenol-inducible promoter)

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189925

   Purpose

   AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SauriABE8e
   

   Use

   AAV

   Mutation

   SauriCas9 D15A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGF1-4eCOL2A1

   Plasmid

   #97210

   Purpose

   Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-egfp-U3-Osp.gRNA

    Plasmid

    #166034

    Purpose

    Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV_Npu-saBE3.9max C-terminal

    Plasmid

    #137180

    Purpose

    Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    Npu-saBE3.9max_C-terminal
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV_Npu-saBE3.9max N-terminal

    Plasmid

    #137179

    Purpose

    Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    Npu-saBE3.9max_N-terminal
    

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    CMV

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-Hsp-eGFP-PGK-mCherry

    Plasmid

    #191862

    Purpose

    Heat inducible eGFP reporter with constitutive mCherry marker

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    eGFP
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    HSPA7 promoter and PGK promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDFDuet-nsp7-nsp8

    Plasmid

    #159092

    Purpose

    Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

    Depositor

    Seth Darst

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Inserts

    NSP7
    NSP8
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. medSIIN

    Plasmid

    #185664

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-pegRNA-H1-nick sgRNA-mCherry

    Plasmid

    #176901

    Purpose

    Expresses prime editing guide RNA and guide RNA in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    mCherry
    gRNA scaffold
    gRNA scaffold
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG, H1, and U6

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hiSIIN

    Plasmid

    #185662

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. midSIIN

    Plasmid

    #185665

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-Hsp-Fluc-PGK-Rluc-mCherry

    Plasmid

    #191863

    Purpose

    Heat inducible Fluc reporter with constitutive Rluc

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    Firefly luciferase Fluc
    Renilla luciferase Rluc
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Promoter

    HSPA7 promoter and PGK promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dimSIIN

    Plasmid

    #185661

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge