We narrowed to 1,529 results for: Flp

1. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

   Plasmid

   #90012

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

   Plasmid

   #97010

   Purpose

   Rosa26 targeting vector for FLP-responsive cassette

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Expression

   Mammalian

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FRT-EBFP-tTAn

   Plasmid

   #172127

   Purpose

   Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174647

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
   

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   CMV (with TetOn)

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-VTKS5-TVA-eGFP-N2cG

   Plasmid

   #175440

   Purpose

   Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

   Depositor

   Gordon Fishell

   Article

   Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

   Insert

   TVA-P2A-eGFP-P2A-N2cG
   

   Use

   AAV and Cre/Lox

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

   Plasmid

   #236159

   Purpose

   Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   NZF-deImmunLink-FRB
   

   Expression

   Mammalian

   Mutation

   T2098L in FRB

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)

   Plasmid

   #236146

   Purpose

   Plasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   NZF-deImmunLink-FRB
   

   Expression

   Mammalian

   Mutation

   N2093W, T2098L in FRB

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

   Plasmid

   #233425

   Purpose

   IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWP170

   Plasmid

   #198792

   Purpose

   Co-expression plasmid for the FLP, PVD neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWP101

    Plasmid

    #198791

    Purpose

    Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

    Plasmid

    #194973

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

    Plasmid

    #194975

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-fDIO-mScarlet-Gphn_P1

    Plasmid

    #194972

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

    Plasmid

    #194974

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174646

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    CMV (with TetOn)

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SapTrap Kit

    Plasmid Kit

    #1000000077

    Purpose

    SapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectors

    Depositor

    Erik Jorgensen

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pFRT-FLAG-HA-(N)-CDK12

    Plasmid

    #231750

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Tags

    Flag-HA-3C

    Expression

    Mammalian

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDragon-p21

    Plasmid

    #155016

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

    Plasmid

    #182925

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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