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Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA03
Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-TEF2
Plasmid#65340Purposeconstruction of TEF2 terminator in HCKan-P receiving vectorDepositorInsertTEF2 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-TDH3
Plasmid#65336Purposeconstruction of TDH3 promoter in HCKan-P receiving vectorDepositorInsertTDH3 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-T-ADH1
Plasmid#65339Purposeconstruction of ADH1 terminator in HCKan-P receiving vectorDepositorInsertADH1 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD125 GFP:POP1
Plasmid#71387Purposelocalization of yeast RNase POP1 (RNase MRP binding protein) in yeastDepositorInsertPOP1 (POP1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterACT1Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HCKan-P-CYC1
Plasmid#65338Purposeconstruction of CYC1 promoter in HCKan-P receiving vectorDepositorInsertCYC1 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS2-Pleum
Plasmid#59992PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tpa1(273-644)
Plasmid#23141DepositorInsertTpa1 C-terminal domain (TPA1 Budding Yeast)
UseTagsHisExpressionBacterialMutationdeleted amino acids 1-272PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SpEAF pBacPAK8
Plasmid#15319DepositorInsertELL associated factor (eaf1 Fission Yeast)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-143
Plasmid#169923PurposeWT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2DepositorInsertEcm2 1-143
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-265
Plasmid#169925PurposeWT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2DepositorInsertEcm2 1-265
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-325
Plasmid#169926PurposeWT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2DepositorInsertEcm2 1-325
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
p101_CRISPRai_PiggyBac
Plasmid#213777PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.DepositorInsertsVPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator
UseCRISPRTagsExpressionMutationPromoterEF1a (EF-1-alpha intron a) and Tet_On_TREAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM65
Plasmid#133654PurposeIntegration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.DepositorInsertp1k-VPH1-mCherry (VPH1 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TAK11
Plasmid#227095PurposeLuciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vectorDepositorInsertCMV Promoter and HIV-1 TAR region
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only