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pDONR223_NRAS_p.Q61K

Plasmid

#81657

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway Cloning

Mutation

Q61K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CRY1_WT

Plasmid

#82264

Purpose

Gateway Donor vector containing CRY1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CRY1 (CRY1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK17_WT

Plasmid

#81776

Purpose

Gateway Donor vector containing CDK17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK17 (CDK17 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

Plasmid

#197265

Purpose

Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_BAX_WT

Plasmid

#82191

Purpose

Gateway Donor vector containing BAX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BAX (BAX Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.G719D

Plasmid

#81708

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

G719D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pDONR223_TP53_WT

Plasmid

#81754

Purpose

Gateway Donor vector containing TP53 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TP53 (TP53 Human)

Use

Gateway Cloning

Mutation

P72R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.E17K

Plasmid

#82089

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway Cloning

Mutation

E17K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HDAC1_WT

Plasmid

#82212

Purpose

Gateway Donor vector containing HDAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HDAC1 (HDAC1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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