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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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plvx-tetO-GFP

Plasmid

#191560

Purpose

Inducible expression of GFP

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

gfp

Use

Lentiviral

Mutation

None

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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B1-5

Plasmid

#111467

Purpose

translational elongation factor Ts

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Ts

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B7-5

Plasmid

#111493

Purpose

translational initiation factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 1

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B7-4

Plasmid

#111492

Purpose

Met-tRNA formyltransferase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA formyltransferase

Tags

6xHis

Expression

Bacterial

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IKZF4-3xHA-pGK-PUR

Plasmid

#107384

Purpose

Lentiviral expression of IKZF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF4 (IKZF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PB-Mycn

Plasmid

#60667

Purpose

PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Mycn (Mycn Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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ptetmCherry_prey_plasmid-2

Plasmid

#34617

Depositor

Lingchong You

Article

Song et al Nat Chem Biol. 2009 Dec;5(12):929-35. Ep

Insert

mCherry

Expression

Bacterial

Promoter

ptet

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Promoter

CamKIIa

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

Plasmid

#199354

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
G protein alpha-q-CFP

Plasmid

#66081

Purpose

This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

Depositor

Catherine Berlot

Article

Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

Insert

G protein alpha-q-EE-CFP (Gnaq Mouse)

Tags

CFP was inserted internally into the alpha-q sequ…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Promoter

HRE-mCMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-C3-beta-spectrin

Plasmid

#202685

Purpose

fluorescent protein tagged beta-spectrin

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

EGFP:SPTB(1-2364) (SPTB Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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