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pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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PGL3-Ogt (-178 to +233 bp)

Plasmid

#79266

Purpose

To identify the Cis and Trans acting elements that control Ogt gene transcription.

Depositor

Steven Jones

Article

Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

Insert

Ogt promoter element (Ogt Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pSN30

Plasmid

#100115

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 EPSM V179T

Plasmid

#27015

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; V179T

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A208S

Plasmid

#27109

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A208S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A213S

Plasmid

#27110

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A213S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Promoter

T7 RNA pol

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4_FUS_G2A_R521H

Plasmid

#26370

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Mutation

R521H

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC WT

Plasmid

#26996

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pTJK6 (miR-513a WT OE in pWCC52)

Plasmid

#254385

Purpose

Express human miR-513a

Depositor

Curt Civin

Article

Kim et al FEBS Open Bio. 2026 Mar 8. doi: 10.1002/

Insert

miR-513a-1 (MIR513A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS31-LV] CMV-MitoTRACER-Donor

Plasmid

#253759

Purpose

Lentiviral MitoTRACER donor construct under CMV Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER Donor

Tags

GFP11 and HA Tag

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS32-LV] EF1a-MitoTRACER-Donor

Plasmid

#253760

Purpose

Lentiviral MitoTRACER donor construct under EF1a Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Tags

GFP11 and HA Tag

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLaco1R1CI*_PLTetO1R1CI*

Plasmid

#250895

Purpose

Cassette 1: Mutated cI protein expressed under PL TetO1 promoter; Cassette 2: Mutated cI protein expressed under PL LacO1 promoter; part of BNeu AS10; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein

Promoter

PL LacO1 and PL TetO1

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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