We narrowed to 59,942 results for: %s

1. FnoCas12a-DR-cr in PBCSK-

   Plasmid

   #126642

   Purpose

   Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET19b-hMED9

   Plasmid

   #15365

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

   Insert

   hMED9 (MED9 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

3. HEL binding scFv D44.1

   Plasmid

   #111718

   Purpose

   Yeast Surface Display of D44.1 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1
   

   Tags

   cmyc tag

   Expression

   Yeast

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVL94 - pOp4:VND7

   Plasmid

   #116016

   Purpose

   destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
   

   Expression

   Plant

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 49535-sgFMR1_E3B

   Plasmid

   #157783

   Purpose

   Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

   Insert

   FMR1 (FMR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MO70-hJunctate - EF-mutant

   Plasmid

   #79597

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

   Insert

   junctate (ASPH Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   77DAD79 to 77AAA79

   Promoter

   CMV

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

8. dmBACCS2VL-IRES-dOrai-IRES-GFP

   Plasmid

   #72897

   Purpose

   Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2VL-IRES-dOrai-IRES-GFP
   

   Tags

   IRES-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LjUBQ:MtGLV6:T35s

   Plasmid

   #164741

   Purpose

   Overexpression of Peptide coding gene (Binary vector)

   Depositor

   Wolf Scheible

   Article

   Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

   Insert

   Medtr3g095180.1
   

   Tags

   Terminator 35S

   Expression

   Plant

   Promoter

   Lotus japonicus Ubiquitin

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LjUBQ:MtGLV2:T35s

    Plasmid

    #164740

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr5g012400.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. L309-myc-seipin-CT

    Plasmid

    #64063

    Purpose

    myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Contains Arg326 to Ser443

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5FRT/TO-p97-RG-mycStrep

    Plasmid

    #31840

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R95G

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

13. ShaCas9

    Plasmid

    #163794

    Purpose

    Expresses ShaCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDE714

    Plasmid

    #103021

    Purpose

    expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHIS4-4.S (HIS4 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_sgACTA2

    Plasmid

    #63712

    Purpose

    Express sgACTA2 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    ACTA2 (ACTA2 Mouse, Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Tags

    6x His Tag

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDK4-luciferase active (880)

    Plasmid

    #86657

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    867 base pairs including up to coding nucleotide …

    Promoter

    CDK5

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1-Barkor (∂CC)

    Plasmid

    #24404

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Barkor (ATG14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 91-180 of the coiled-coil…

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only