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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-N22p-3XRFPnls

Plasmid

#75387

Purpose

N22p-3XRFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

N22p

Use

Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Scrambled control shRNA

Plasmid

#40625

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Scrambled control shRNA

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP wt

Plasmid

#41387

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Expression

Bacterial

Promoter

lac

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5M-TIMP3

Plasmid

#31715

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TIMP3 (TIMP3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pSH100 (YCplac33 MET25pro MCP-mCherry)

Plasmid

#45930

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-mCherry

Tags

mCherry

Expression

Yeast

Promoter

Met25

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-tat

Plasmid

#22502

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Transactivator of Transcription

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
GST HP1 alpha

Plasmid

#24074

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
p3998 pGEX HHR23A

Plasmid

#10864

Depositor

Peter Howley

Article

Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

Insert

HHR23A (RAD23A Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
Epsin1-ECFP

Plasmid

#21066

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin 1 (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
Epsin1 deltaUIM-ECFP

Plasmid

#20973

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin1 deltaUIM (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

June 15, 2009

Availability

Academic Institutions and Nonprofits only
pC1-HyPer-2

Plasmid

#42211

Purpose

A genetically encoded sensor for H2O2 with expanded dynamic range.

Depositor

Vsevolod Belousov

Article

Markvicheva et al Bioorg Med Chem. 2011 Feb 1;19(3):1079-8

Insert

HYPER 2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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Nampt-PET28a

Plasmid

#25630

Depositor

Cynthia Wolberger

Article

Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

Insert

Nampt (Nampt Mouse)

Tags

His6

Expression

Bacterial

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pLSSmKate1-N1

Plasmid

#31866

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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