Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 15,594 results for: LEA

Showing: 10801 - 10820 of 15594 results

  1. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

    Enlarge

  2. pHelper_O

    Plasmid
    #173783
    Purpose
    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    186 Integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    E59G - please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173783-plasmid-map-sequence-id-342367

    Enlarge

  3. RECQL5 (RECQL5A-c011)

    Plasmid
    #98231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RECQL5A (RECQL5 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98231-plasmid-map-sequence-id-193411

    Enlarge

  4. human KANK1 Ankyrin Repeats pET151

    Plasmid
    #164053
    Purpose
    Expresses human KANK1 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164053-plasmid-map-sequence-id-327672

    Enlarge

  5. pGBW-m4046464

    Plasmid
    #148970
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148970-plasmid-map-sequence-id-297202

    Enlarge

  6. pICSL50016

    Plasmid
    #50315
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    Use
    Plant expression
    Tags
    Expression
    Mutation
    Codon-optimised for A. thaliana. BsaI/BbsI sites …
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50315-plasmid-map-sequence-id-75072

    Enlarge

  7. 5FAI (EMG1)

    Plasmid
    #79519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EMG1 (EMG1 Human)
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79519-plasmid-map-sequence-id-150117

    Enlarge

  10. pSDMA66

    Plasmid
    #128355
    Purpose
    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do
    Insert
    Noureothricin (NAT)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128355-plasmid-map-sequence-id-254590

    Enlarge

  14. 5THA (Gemin5)

    Plasmid
    #101890
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Gemin5 (GEMIN5 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, Thrombin cleavage
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101890-plasmid-map-sequence-id-194723

    Enlarge

  16. pGBW-m4258326

    Plasmid
    #154793
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154793-plasmid-map-sequence-id-311545

    Enlarge

  17. pGBW-m4046574

    Plasmid
    #148976
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148976-plasmid-map-sequence-id-299914

    Enlarge

  20. WDR5

    Plasmid
    #36900
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    mutation version of plasmid #60121 3UR4
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10801 - 10820 of 15594 results