We narrowed to 15,822 results for: LEA

1. pTP1112

   Plasmid

   #104158

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FnR8-10

   Plasmid

   #182569

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEX-LV

   Plasmid

   #12120

   Depositor

   Inder Verma

   Article

   Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

   Insert

   Matrix Metalloproteinase 2 (Mmp2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   noncatalytic fragment of MMP2 comprised of the fi…

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pET16better_H10_HRV3C_chitinase

   Plasmid

   #175091

   Purpose

   Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

   Depositor

   Jay C Dunlap

   Article

   Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

   Insert

   chitinase
   

   Use

   Tags

   10X His, HRV-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAPV-DsRed2-ER-5

   Plasmid

   #170570

   Purpose

   Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Parp14-c012

   Plasmid

   #173099

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   W1003-A1190

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Parp14-c010

   Plasmid

   #173100

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   F1208-G1388

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

   Plasmid

   #131034

   Purpose

   Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRB (Tcrb Mouse)
   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_HaloTag9_LamB1

    Plasmid

    #175538

    Purpose

    Expression of HaloTag9 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-GR-ICUE2

    Plasmid

    #173017

    Purpose

    Improved green-red FRET based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-ICUE2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - TR

    Plasmid

    #89621

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Thioredoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NES-mCherry-P4Mx1

    Plasmid

    #108143

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mini-donor - SOX17-EGFP

    Plasmid

    #206048

    Purpose

    this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_Lyn11-HaloTag9

    Plasmid

    #175543

    Purpose

    Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4-myc-nuc (w556-1)

    Plasmid

    #20026

    Purpose

    Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNH-NanoLuc

    Plasmid

    #173075

    Purpose

    Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MR015-H11-L2-TALEN

    Plasmid

    #51554

    Purpose

    Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    H11-L2 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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