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pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S61A

Plasmid

#33087

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S61A

Promoter

CMV

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only
pLPC MYC mTel2

Plasmid

#22803

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (Telo2 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a1(2nd methione)

Plasmid

#15746

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1-2nd M (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
Axin GID 5-6 LP/pCS2MT

Plasmid

#16302

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 5-6 LP (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Mutation

Amino acids 380-404, L to P mutation

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf98

Plasmid

#12786

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf98

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
p3004 pGEM E6-AP 76kD

Plasmid

#10857

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

76kD: amino acids 213-865

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
p3012 pGEM E6-AP C-A

Plasmid

#8645

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

active site cyteine codon mutated to encode alani…

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pIVT_goldDn29-dCas9-P2A-GFP

Plasmid

#247167

Purpose

In vitro transcription of human codon optimized fusion of goldDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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snuABE4.1

Plasmid

#246013

Purpose

snuABE4.1 composed of PhADAR(E438Q, I536S, E637F) with 10a.a truncation fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.

Depositor

Sangsu Bae

Article

Im et al bioRxiv 2025.07.19.664718

Insert

Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)

Expression

Mammalian

Mutation

E438Q, I536S, E637F, with 10 amino acids truncati…

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HCT159_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

Plasmid

#233558

Purpose

Direct-expressing iCre297T AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

iCre297T

Use

AAV

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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HCT46_rAAV-eHGT_1188m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#223943

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP11-LRRK2_G2019S

Plasmid

#231175

Purpose

Expresses GFP-11-LRRK2 G2019S in mammalian cells

Depositor

Samara Reck-Peterson

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

GFP11-LRRK2 (G2019S) (LRRK2 Human)

Tags

GFP11

Expression

Mammalian

Mutation

G2019S

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-mGFP-SNX9

Plasmid

#223632

Purpose

Lentiviral expression of human SNX9 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SNX9 (SNX9 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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yTREX

Plasmid

#177306

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-mKate2

Plasmid

#220607

Purpose

Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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