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pENTR1A mKate2-hu_ncOGT K842M

Plasmid

#154284

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

Depositor

Suzanne Walker

Publication

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway Cloning

Tags

2x FLAG and mKate2

Mutation

K842M mutation (Catalytic Inactive)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP4

Plasmid

#158355

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP4 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Nov. 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCellFree_G06_ZIKV NS5

Plasmid

#145358

Purpose

Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pK7WG2-mitoTAL1-orf125

Plasmid

#158736

Purpose

Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

Depositor

Shin-ichi Arimura

Publication

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

mitoTALEN left and right to target orf125 (orf138)

Use

TALEN

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pK7WG2-mitoTAL2-orf125

Plasmid

#158737

Purpose

Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

Depositor

Shin-ichi Arimura

Publication

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

mitoTALEN left and right to target orf125 (orf138)

Use

TALEN

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR1A_Mavs (open)

Plasmid

#160099

Purpose

Expresses murine Mavs in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Mavs (Mavs Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmMavs_V5

Plasmid

#160100

Purpose

Expresses murine Mavs in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Mavs (Mavs Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

Plasmid

#159680

Purpose

Mammalian protein expression of KRAS4a-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4a (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

Plasmid

#159687

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

Depositor

Dominic Esposito

Publication

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Insert

His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Promoter

acta1

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP9

Plasmid

#158360

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP9 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf9c

Plasmid

#158379

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9c

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 M

Plasmid

#158369

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 M (M )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf10

Plasmid

#158380

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf10 (ORF10 )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP14

Plasmid

#158365

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP14 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP1

Plasmid

#158352

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP1 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP2

Plasmid

#158353

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP2 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

Plasmid

#156411

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

Depositor

Joshua Wythe

Publication

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS11D-G418

Plasmid

#158477

Purpose

Constitutive lentiviral expression of human TMPRSS11D.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS11D (TMPRSS11D Human)

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-short_BrokenHeart (no BC)

Plasmid

#86950

Purpose

AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

Depositor

Joseph Dougherty, Rob Mitra

Publication

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

Brokenheart construct

Use

AAV

Expression

Mammalian

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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