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pCBS-6756

Plasmid

#249328

Purpose

Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

EPEC DarT2(G49D)-nSpCas9(D10A)

Tags

3X Flag tag

Expression

Mammalian

Mutation

DarT2(G49D) and nSpCas9(D10A)

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor NTRK1-P2A-tdTomato

Plasmid

#246653

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human NTRK1 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by NTRK1 homology regions (NTRK1 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Mutation

tdTomato was altered to contain a silent SAL1 res…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-TOP3B (WT)-3xFLAG

Plasmid

#249681

Purpose

Expresses human TOP3B (wildtype) with a C-terminal 3xFLAG tagged in mammalian cells

Depositor

Michael Kearse

Article

Warrick et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

human TOP3B (TOP3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-fibrillarin-FRT

Plasmid

#247341

Purpose

FLP/FRT based mammalian expression vector for EGFP-fibrillarin driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Imai et al Mol Biol Cell. 2017 Nov 7;28(23):3349-33

Insert

fibrillarin (FBL) (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N178/298/358Q)

Plasmid

#214804

Purpose

Mammalian expression of human MPL(N178/298/358Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N178/298/358Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR del52-linker-Venus-KDEL

Plasmid

#214786

Purpose

Mammalian expression of human del52-linker-Venus-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-linker-Venus-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR ins5-linker-Venus-KDEL

Plasmid

#214787

Purpose

Mammalian expression of human CALR ins5-linker-Venus-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-linker-Venus-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR del52-linker-Venus

Plasmid

#214788

Purpose

Mammalian expression of human CALR del52-linker-Venus

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR ins5-linker-Venus

Plasmid

#214789

Purpose

Mammalian expression of human CALR ins5 -linker-Venus

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-KDEL

Plasmid

#214784

Purpose

Mammalian expression of human CALR del52-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-KDEL

Plasmid

#214785

Purpose

Mammalian expression of human CALR ins5-KDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-KDEL (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE-hGHpA

Plasmid

#246243

Purpose

Cre dependent flag-tagged Rpl22l1.

Depositor

D. James Surmeier

Article

Tanimura et al Neuron. 2019 Feb 6;101(3):444-458.e6. do

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

Myc-FLAG-T2A-tdTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available Since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA

Plasmid

#227683

Purpose

To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567A

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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