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pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B3

Plasmid

#172844

Purpose

CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mTurquoise2

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-GAS41

Plasmid

#15428

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

GAS41 (Yeats4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
c-myc promoter (Frag A)

Plasmid

#16605

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag A) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragA (XmnI->KpnI). The KpnI site is at -1194 …

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR V2 sgMTHFD1L_1

Plasmid

#106316

Purpose

Express Cas9 and sgRNA targeting MTHFD1L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD1L

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-HsUBB

Plasmid

#69562

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (UBB Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

Plasmid

#197968

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-RrA3F F130L

Plasmid

#138341

Purpose

Mammalian expression plasmid for BE4-RrA3F F130L

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-RrA3F F130L

Use

Synthetic Biology

Tags

BP-NLS

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B4

Plasmid

#172845

Purpose

CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mNeonGreen

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEE531

Plasmid

#149290

Purpose

T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKC158

Plasmid

#134242

Purpose

CENP-A Halo expression construct

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP-A-Halo tagging construct (CENPA Human)

Use

Human targeting

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p6895 PHAGE-P CMVT N-HA-hE6AP I wt

Plasmid

#37601

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I wt (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV tet regulatable

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_703128

Plasmid

#224394

Purpose

For expression of the type I CBASS system encoded by Escherichia coli 703128

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_BWH59

Plasmid

#224393

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BWH59

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

2TM_EcCdnD

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE479

Plasmid

#153270

Purpose

M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT1B

Plasmid

#53108

Purpose

Expression of Drosophila 5-HT1B

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT1B (5-HT1B Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only