We narrowed to 63,295 results for: cha;
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Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNX19-PX (528-664) (mouse)
Plasmid#119100PurposeBacterial expression of mouse phox homology (PX) domain, SNX19-PX (528-664) (mouse)DepositorAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFIN-mIRBP156-CHER-RK-GFP-WPRE
Plasmid#44593DepositorInsertsIRBP156-CHER
RK-GFP
UseLentiviralPromoterhuman rhodopsin kinase (pos 3723-4019) and murine…Available SinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40
Plasmid#180758PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)DepositorInsertα-COPI-WD40
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Leu181Lys, Leu…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-6-C, 16-C
Plasmid#124689PurposeTuning expression of PD-1DepositorAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
IFITM3-N21del-V93-TAG
Plasmid#122480Purposeexpresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
ExpressionMammalianMutationChanged Valine 93 to amber codon and deleted amin…Available SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E DN Kif5a
Plasmid#105973Purpose3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bpDepositorAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_Lys322Arg
Plasmid#105862PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationLys322Arg in constant region of IgG1 heavy chainPromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM4663
Plasmid#85126PurposeNSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.DepositorInsertPkaiB-luciferase
ExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
exon L-firefly
Plasmid#81006Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
Tagsfirefly luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeQ-GFP]
Plasmid#153540PurposeAAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 DEL NTERM
Plasmid#58894PurposeExpresses GFP tagged TEM4 with N terminus deletedDepositorInsertTEM4 (ARHGEF17 Human)
TagsEGFPExpressionMammalianMutationDeleted N Terminus up to aa 1002PromoterCMVAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBScMXT + mSlo1 MBr5/23
Plasmid#16151DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
UseXenopus oocyte expressionMutationN/AAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-1
Plasmid#110366PurposeMammalian expression of HisA-tagged HOXC6-1DepositorAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta 6X mutant GFP
Plasmid#128823PurposeTo visualize Doc2beta 6X mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
TagsEGFPExpressionMammalianMutationD163A, D218A, D220N, D303A, D357A, D359APromoterEF-1alphaAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM4731
Plasmid#85127PurposeNSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.DepositorInsertPkaiBC-KaiB
ExpressionBacterialAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SH3PXD2A S974A
Plasmid#69814PurposeExpresses M2-SH3PXD2A in mammalian cellsDepositorInsertSH3 and PX domain-containing protein 2A (SH3PXD2A Human)
TagsFLAGExpressionMammalianPromoterSV40 early promoterAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PI3KC2alpha-PX (1405-1545)
Plasmid#119119PurposeBacterial expression of human phox homology (PX) domain, PI3KC2alpha-PX (1405-1545)DepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-1-A, 5-A
Plasmid#124682PurposeTuning expression of PD-1DepositorAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only