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pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

Plasmid

#169469

Purpose

Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEUT RUVBL1 Delete

Plasmid

#159144

Purpose

Expression and protien purification of mutated RUVBL1

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL1 (RUVBL1 Human)

Expression

Bacterial

Mutation

amino acid 1-463, Glu134-Glu237 mutated to GPPG

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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lentiGuide-chr20-49345720-gRNA1

Plasmid

#232623

Purpose

Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-chr20-49345720-gRNA2

Plasmid

#232624

Purpose

Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-chr20-49345720-gRNA3

Plasmid

#232625

Purpose

Lentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-2

Plasmid

#210140

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

Plasmid

#169468

Purpose

Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

Plasmid

#169466

Purpose

Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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BM28 ΔlysU

Bacterial Strain

#201048

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Kanamycin

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-E210A

Plasmid

#203697

Purpose

expresses ATP10B with the catalytic mutation E210A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

E210A

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-D433N

Plasmid

#203696

Purpose

expresses ATP10B with the catalytic mutation D433N

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

D433N

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUC_hU6-shRKIP_BFP

Plasmid

#199216

Purpose

Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Promoter

human U6

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available Since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

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ERBB3 gRNA (BRDN0001149441)

Plasmid

#77480

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB3 (ERBB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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