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98.a_pSTC0-zeo

Plasmid

#202332

Purpose

Design 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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98.b_pSTC0-zeo

Plasmid

#202333

Purpose

Design 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL854

Plasmid

#214254

Purpose

Expression eTd-CBE in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF_TypeIC_target_sfGFP_invivo

Plasmid

#196399

Purpose

expresses CRISPR Cascade Type IC target sequence for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

type IC dsDNA target

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-Clover

Plasmid

#126572

Purpose

Multisite gateway vector for 3' tagging with Clover. Parton lab clone DJZ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Clover

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb

Plasmid

#141067

Purpose

Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Synthetic Biology

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPEE38

Plasmid

#128378

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the HYG resistance marker.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Expression

Mammalian

Promoter

U6

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE41

Plasmid

#128381

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the HYG resistance marker.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE40

Plasmid

#128380

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the HYG resistance marker.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE37

Plasmid

#128377

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the HYG resistance marker.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE31

Plasmid

#128371

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE39

Plasmid

#128379

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the HYG resistance marker.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE29

Plasmid

#128369

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE28

Plasmid

#128368

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE30

Plasmid

#128370

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PP310

Plasmid

#78979

Purpose

245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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