Addgene

We narrowed to 2,318 results for: PUC19

Showing: 1081 - 1100 of 2318 results

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pSW039

Plasmid

#140035

Purpose

KO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Yellow fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

cLac143

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSW036

Plasmid

#140034

Purpose

KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Yellow fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

cpt

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pG106 - KA

Plasmid

#178041

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSW071

Plasmid

#140037

Purpose

PCC 11901 fadD locus KO with a kanamycin resistance cassette

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Kanamycin resistance gene

Use

Gene knockout in cyanobacteria

Tags

Expression

Mutation

Promoter

KanR

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKSJ331

Plasmid

#103063

Purpose

Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

Depositor

Alan Finkelstein, Karen Jakes

Article

Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

Insert

Colicin E1 and E1 immunity protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

colicin promotor from ColE1

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDD285

Plasmid

#66826

Purpose

mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mKate2

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B16-TEF(272)

Plasmid

#44375

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(272) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(272)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B16-TEF(504)

Plasmid

#44371

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(504) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(504)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(136)

Plasmid

#44380

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(136) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(136)

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pADH110

Plasmid

#90982

Purpose

NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

NAT 2 of 2
pSNR52

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

A. niger glucoamylase promoter (glaAp)4

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Use

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only