We narrowed to 2,878 results for: 623

1. p663-UBC-miniTurbo-V5-CDK14_IDG-K

   Plasmid

   #158172

   Purpose

   Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK14 (CDK14 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

   Plasmid

   #71761

   Purpose

   mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

   Depositor

   Liqun Luo

   Article

   Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

   Inserts

   mGFP
   Synaptophysin
   

   Use

   AAV

   Tags

   Palmitoylation signal and mRuby

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TrkB Acceptor

   Plasmid

   #83953

   Purpose

   FRET acceptor for TrkB mEGFP

   Depositor

   Ryohei Yasuda

   Article

   Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

   Insert

   Phospholipase C gamma 1 (Plcg1 Mouse)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. tdTomato-LacI

   Plasmid

   #183921

   Purpose

   Lac repressor labeled with tdTomato; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdTomato-LacI (lacI Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ-Wt-PTEN

   Plasmid

   #206982

   Purpose

   Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCool-mRbx1-His-hCul1-CTP

   Plasmid

   #29519

   Depositor

   Ning Zheng

   Insert

   Rbx1 CUL1 (CUL1, Rbx1 Mouse, Human)
   

   Use

   Tags

   GST, His (before thrombin), and Thrombin Cut Site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

7. mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

   Plasmid

   #214989

   Purpose

   Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-PAK2 binding domain

   Plasmid

   #83951

   Purpose

   FLIM acceptor for mEGFP Rac1

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   Pak2 (PAK2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa60-113 only; S74A F84A

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE

   Plasmid

   #120395

   Purpose

   pAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

   Insert

   Scrambled shRNA
   

   Use

   AAV and RNAi

   Tags

   mCherry.3XFLAG

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVV099

    Plasmid

    #215305

    Purpose

    Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

    Plasmid

    #214994

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHKMC1: Empty sgRNA for Cloning

    Plasmid

    #67720

    Purpose

    Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

    Depositor

    Monica Colaiacovo

    Article

    Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    PU6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK313

    Plasmid

    #59570

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK317

    Plasmid

    #59571

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. plenti-UBC-Myc-gate-pGK-HYG

    Plasmid

    #107395

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMVV207

    Plasmid

    #215343

    Purpose

    gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    S. stutzeri nifL gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMVV205

    Plasmid

    #215342

    Purpose

    Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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