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Showing: 1081 - 1100 of 26848 results

  1. pLY320

    Plasmid
    #184161
    Purpose
    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184161-plasmid-map-sequence-id-376082

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  2. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  3. pAAV Syn Barcode18

    Plasmid
    #226191
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode18
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226191-plasmid-map-sequence-id-447872

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  4. pGBScomp High

    Plasmid
    #223202
    Purpose
    Group B Streptococcus High Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223202-plasmid-map-sequence-id-451880

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  5. pGBScomp Low

    Plasmid
    #223203
    Purpose
    Group B Streptococcus Low Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223203-plasmid-map-sequence-id-451879

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  6. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    201402-plasmid-map-sequence-id-394260

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  7. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188673-plasmid-map-sequence-id-375111

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  8. SGEP-sh-Hs-PHGDH-1967

    Plasmid
    #188672
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188672-plasmid-map-sequence-id-375054

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  9. pENTRY4_ Pi21

    Plasmid
    #126904
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126904-plasmid-map-sequence-id-249827

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  10. pICSL4723_ Pi21

    Plasmid
    #126892
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126892-plasmid-map-sequence-id-249677

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  11. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Depositor
    Kris Wood, David Sabatini
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  13. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

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  16. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid
    #185061
    Purpose
    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185061-plasmid-map-sequence-id-376807

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  19. pVG143

    Plasmid
    #227754
    Purpose
    Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al bioRxiv 2024.02.28.582517
    Insert
    EL222 codon-optimized for budding yeast
    Use
    Tags
    Expression
    Yeast
    Mutation
    Leu87Ile
    Promoter
    PGK1pr
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    227754-plasmid-map-sequence-id-458037

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Showing: 1081 - 1100 of 26848 results