We narrowed to 9,426 results for: ef1

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p416TEF1 Cystector (Cysteine biosensor)

Plasmid

#250654

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pGA1-EF1α-sfGFP150TAG-4xtRNA

Plasmid

#251517

Purpose

Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

sfGFP150TAG

Tags

His6 and V5

Expression

Mammalian

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

EF1alpha

Available Since

March 12, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -NaChBac

Plasmid

#246997

Purpose

Can be used to generate AAV virus that will express NaChBac from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

NaChBac

Use

AAV

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2

Plasmid

#246998

Purpose

Can be used to generate AAV virus that will express human KCNJ2 from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

hKCNJ2 (KCNJ2 Human)

Use

AAV

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1α-EGFP-WPRE

Plasmid

#250407

Purpose

Lentiviral expression of EGFP under EF1α core promoter.

Depositor

Stefan Kubicek

Article

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1α-PPAT-WPRE

Plasmid

#250408

Purpose

Lentiviral expression of PPAT under EF1α core promoter.

Depositor

Stefan Kubicek

Article

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

PPAT (PPAT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1

Plasmid

#249270

Purpose

Expression of GLI1

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA + 3xGS linker

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-RPA194

Plasmid

#247348

Purpose

Expresses HaloTag-RPA194

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-fibrillarin-FRT

Plasmid

#247341

Purpose

FLP/FRT based mammalian expression vector for EGFP-fibrillarin driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Imai et al Mol Biol Cell. 2017 Nov 7;28(23):3349-33

Insert

fibrillarin (FBL) (FBL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-coilin-FRT

Plasmid

#247448

Purpose

FLP/FRT based mammalian expression vector for EGFP-coilin, driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Itoh et al Life Sci Alliance. 2021 Feb 3;4(4):e2020

Insert

coilin (COIL) (COIL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-HaloTag-FRT

Plasmid

#247338

Purpose

Expresses H2B-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-sgRXRa4-1; EF1a-mScarlet

Plasmid

#242889

Purpose

For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

Depositor

Elaine Fuchs

Article

Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

Insert

sgRNA targeting Rxra

Use

CRISPR and Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-2-EF1a-LibVec

Plasmid

#239593

Purpose

expresses non-targeting (NT) guide#2

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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