We narrowed to 15,823 results for: LEA

1. csr-1_son-1_mCherry

   Plasmid

   #191742

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLS-LSSmKate2-N1

   Plasmid

   #31871

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

   Promoter

   Available since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJExpress414_GmPOPB-S577A

   Plasmid

   #92235

   Purpose

   Express S577A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-S577A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Serine 577 to Alanine

   Promoter

   T7 promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIS.MTRIA-PRLH

   Plasmid

   #184618

   Purpose

   For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-PRLH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046422

   Plasmid

   #145515

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MTK4a_003

   Plasmid

   #123817

   Purpose

   Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EPHB3

   Plasmid

   #28165

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHB3 (EPHB3 Human)
   

   Use

   Tags

   His tag with TEV cleavage and Honeybee melittin S…

   Expression

   Bacterial

   Mutation

   Ligand binding domain (residues 39-211).

   Promoter

   Available since

   March 18, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

8. RECQL5 (RECQL5A-c104)

   Plasmid

   #98234

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RECQL5A (RECQL5 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos4b-LaminA-C-18

   Plasmid

   #57511

   Purpose

   Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mEos4b

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. HLTF

    Plasmid

    #28152

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    helicase-like transcription factor (HLTF Human)
    

    Use

    Tags

    thrombin cleavage

    Expression

    Bacterial

    Mutation

    Contains HIRAN domain (residues 51 to 171).

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pGBW-m4046173

    Plasmid

    #149126

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046397

    Plasmid

    #145479

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGC4780

    Plasmid

    #113408

    Purpose

    Translation reporter with inducible translation coupling (high native GFP expression)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    158_33121123_1
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046506

    Plasmid

    #145560

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046486

    Plasmid

    #145506

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046351

    Plasmid

    #145449

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDGE62

    Plasmid

    #79444

    Purpose

    recipient plasmid [single nuclease]; p35S:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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