We narrowed to 15,981 results for: LEA

1. KEAP1 (KEAP1A-c029)

   Plasmid

   #110247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KEAP1 (KEAP1 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFBNb-cALFA

   Plasmid

   #159990

   Purpose

   For the protein expression with ALFA tag at C-terminus in insect cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Insect

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA-FRT-FAK30

   Plasmid

   #59121

   Purpose

   FAK fret sensor with 30 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DOT1L

   Plasmid

   #36196

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DOT1L (GeneScript Synthesized) (DOT1L Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acid residues 1-420

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. NES-iRFP-P4Mx1

   Plasmid

   #108144

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. JMJD1CA-c089

   Plasmid

   #79644

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1C (JMJD1C Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Mutation

   Contains D10A and H840A mutations

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR_full-site

   Plasmid

   #186277

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2-FAK30

   Plasmid

   #59131

   Purpose

   Cloning vector for FAK fret sensor with 30 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MXS_P2A

    Plasmid

    #62433

    Purpose

    MXS_chaining vector with P2A

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Self-cleaving peptide
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA-FRT-FAK10

    Plasmid

    #59119

    Purpose

    FAK fret sensor with 10 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. csr-1_son-1_mTagBFP2

    Plasmid

    #191746

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and mTagBFP2

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. MECR

    Plasmid

    #38850

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MECR (Mecr Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCMV10-3xFLAG-prs-MCP1

    Plasmid

    #184341

    Purpose

    Express yeast MCP1 in Expi293F cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Yeast MCP1 (MCP1 Budding Yeast)
    

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-FAK0

    Plasmid

    #59128

    Purpose

    Cloning vector for FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. csr-1_son-1_vsfGFP9

    Plasmid

    #191747

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and vsfGFP-9

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJOG824

    Plasmid

    #105401

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    SV40 nuclear localization signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    March 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge