We narrowed to 18,398 results for: Mut

1. pBAD.Sigma32.I54N

   Plasmid

   #59982

   Purpose

   Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

   Depositor

   Jeffery Kelly

   Article

   Zhang et al ACS Chem Biol. 2014 Jul 22.

   Insert

   Sigma32 I54N
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I54N

   Promoter

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

   Plasmid

   #175570

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. G to C …

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Casp3 C163A-myc

   Plasmid

   #11814

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C163A

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. GAG-Pan

   Plasmid

   #99640

   Purpose

   EGFP-mCherry Met71GAG reporter for methionine misincorporation

   Depositor

   Tao Pan

   Article

   Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

   Insert

   mCherry
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Met71 mutated to GAG (Glu)

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GAA-Pan

   Plasmid

   #99639

   Purpose

   EGFP-mCherry Met71GAA reporter for methionine misincorporation

   Depositor

   Tao Pan

   Article

   Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

   Insert

   mCherry
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Met71 mutated to GAA (Glu)

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT10

   Plasmid

   #223382

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TALED_Right-ND1-AD

   Plasmid

   #183900

   Purpose

   Expresses TALED_Right-ND1-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

   Plasmid

   #20298

   Purpose

   Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   channelrhodopsin-2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized ChR2 gene with histidine 134 changed to…

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALED_Left-ND1-E1347A

   Plasmid

   #183895

   Purpose

   Expresses TALED_Left-ND1-E1347A in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQAT11

    Plasmid

    #223383

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT08

    Plasmid

    #223380

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQFT-mNGsacB

    Plasmid

    #203676

    Purpose

    pQFT with mNeonGreen and sacB under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX hCD4

    Plasmid

    #14614

    Depositor

    Dan Littman

    Article

    Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

    Insert

    CD4 (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSAM_Bt

    Plasmid

    #112497

    Purpose

    Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

    Depositor

    Jeffrey Gordon, Andrew Goodman

    Article

    Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

    Inserts

    INSeq-adapted mariner transposon
    Himar1C9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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