We narrowed to 13,693 results for: crispr cas9

1. pAAV-i53

   Plasmid

   #92170

   Purpose

   recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

   Depositor

   Daniel Durocher

   Article

   Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

   Insert

   i53
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   UbvG08 with I44A mutation and no terminal Glycines

   Promoter

   CMV

   Available Since

   July 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pVSV-G

   Plasmid

   #138479

   Purpose

   Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   VSV-G envelop protein
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE Dlg4-GFP KI

   Plasmid

   #131477

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/T0-Flag-RECQL5

   Plasmid

   #222620

   Purpose

   Mammalian expression vector of flag-tagged RECQL5

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   RECQL5 (RECQL5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHLS-EF1a-FKBP12-Gag(HIV)

   Plasmid

   #138476

   Purpose

   Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   HIV Gag
   

   Tags

   FRBP12 and Lynn Myristolation site

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE GFP-Actb KI

   Plasmid

   #131479

   Purpose

   Endogenous tagging of βactin: N-terminal (amino acid position: D2)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Actb, Actb Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE Gria1-GFP KI

   Plasmid

   #131489

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/T0-Flag-BRIP1

    Plasmid

    #222619

    Purpose

    Mammalian expression vector of flag-tagged BRIP1

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    BRIP1 (BRIP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pORANGE GFP-CACNA1A KI

    Plasmid

    #131480

    Purpose

    Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1a, Cacna1a Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE mEos3.2-Camk2a KI

    Plasmid

    #131493

    Purpose

    Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and mEos3.2 donor (Camk2a, Camk2a Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-46: MYL7-mEGFP

    Plasmid

    #114413

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-Arpc5 KI

    Plasmid

    #131503

    Purpose

    Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Mutation

    G12V

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE CACNG2-GFP KI

    Plasmid

    #131504

    Purpose

    Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng2, Cacng2 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT/T0-EGFP-GNB1L

    Plasmid

    #222621

    Purpose

    Mammalian expression vector of flag-tagged GNB1L

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    GNB1L (GNB1L Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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