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pYTK087

Plasmid

#65194

Purpose

Encodes LEU2 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 3' Homology

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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LIIbeta R 1-2

Plasmid

#54357

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-H2-M9

Plasmid

#15868

Depositor

Peter Mombaerts

Publication

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

H2-M9 (H2-M9 Mouse)

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pDE43-MCKq26

Plasmid

#27336

Depositor

Dirk Schnappinger

Publication

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag26

Use

Gateway Cloning

Mutation

N/A

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV2.NN

Plasmid

#242202

Purpose

AAV packaging plasmid expressing Rep/Cap genes for production of AAV2.NN

Depositor

Stylianos Michalakis

Publication

Pavlou et al EMBO Mol Med. 2021 Apr 9;13(4):e13392. d

Inserts

AAV2 rep
AAV2.NN cap

Use

AAV

Mutation

replaced R588 with AAANNPTPSRAAR

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV2.GL

Plasmid

#242201

Purpose

AAV packaging plasmid expressing Rep/Cap genes for production of AAV2.GL

Depositor

Stylianos Michalakis

Publication

Pavlou et al EMBO Mol Med. 2021 Apr 9;13(4):e13392. d

Inserts

AAV2 rep
AAV2.GL cap

Use

AAV

Mutation

replaced R588 with AAAGLSPPTRAAR

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2652

Plasmid

#252582

Purpose

TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Publication

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1-WFR with AtCHLl1 gRNA4

Use

CRISPR

Expression

Plant

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Publication

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mScarlet-I-QtSig

Plasmid

#236775

Purpose

Expresses the red fluorescent protein mScarlet-I fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Publication

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

HA_DD-N_mScarlet-I_QtSig

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Publication

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_FluB

Plasmid

#175956

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]

Depositor

Paul Freemont

Publication

Unruh et al medRxiv 2024.04.17.24305965

Insert

NEP gene

Expression

Bacterial

Promoter

T7

Available since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGEARBOCS-v0-Gfap- TagIN-HA

Plasmid

#196491

Purpose

To tag endogenous mouse GFAP with HA

Depositor

Cagla Eroglu

Publication

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Inserts

HA
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mOrange in pBSDONR P4r-P2

Plasmid

#121529

Purpose

For making C-terminal mOrange fusions using multisite Gateway cloning

Depositor

Roger Innes

Insert

mOrange

Expression

Bacterial

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTG131

Plasmid

#198640

Purpose

Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

Depositor

David Bikard

Publication

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

Cas9

Expression

Bacterial

Promoter

pPhlF

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEY21

Plasmid

#191027

Purpose

ggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-3

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY88

Plasmid

#191081

Purpose

ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-1

Expression

Worm

Mutation

None

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSB3K3-101-phzAG

Plasmid

#165617

Purpose

Constitutive expression of phzAG operon for phenazine-1-carboxylic acid production

Depositor

Martin Buck

Insert

phzABCDEFG

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDmVasa

Plasmid

#165783

Purpose

Drosohphila vasa germline promoter and 5'UTR

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vasa promoter

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xY-1

Plasmid

#134807

Purpose

IPTG inducible expression of Y-TAGA qtRNA

Depositor

Ahmed Badran

Publication

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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