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pHygroR-GMRWhite

Plasmid

#165886

Purpose

Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

Plasmid

#137859

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRVV655

Plasmid

#119019

Purpose

Drives TP901-1 recombinase expression in the D. melanogaster germ line

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 recombinase

Tags

SV40 NLS

Expression

Insect

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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OA959C

Plasmid

#104968

Purpose

Expresses anti-ZIKV transgene in Ae. aegypti

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

Insert

Anti-Zika Transgene

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Expression

Mammalian

Promoter

U6

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP pA

Plasmid

#80814

Purpose

nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

nlsGFPpA 3'E

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pIM35

Plasmid

#74644

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12m

Plasmid

#69943

Purpose

pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

pSCKtheoRaj12m

Use

Synthetic Biology; Psc101 origin of replication

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMM750

Plasmid

#68899

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSβ

Plasmid

#68283

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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LI dy Esp3I-ccdB C-D

Plasmid

#54460

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

ccdB C-D

Use

Cloning vector

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
LI dy Esp3I-lacZ C-D

Plasmid

#54458

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

lacZ C-D

Use

Cloning vector

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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LI dy Esp3I-ccdB A-B

Plasmid

#54459

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

ccdB A-B

Use

Cloning vector

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
LI dy Esp3I-lacZ A-B

Plasmid

#54457

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

lacZ A-B

Use

Cloning vector

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D GFP c1

Plasmid

#54414

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

sGFP

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D T-Saphire c1

Plasmid

#54416

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D mOrange c3

Plasmid

#54419

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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