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pC008 - pBR322 with tetR-inducible RFP

Plasmid

#79157

Purpose

inducible RFP expression vector

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

TetR + RFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMPRAv3:∆luc:∆xbaI

Plasmid

#109035

Purpose

MPRA v3 empty backbone

Depositor

Pardis Sabeti, Ryan Tewhey

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Type

Empty backbone

Expression

Mammalian

Promoter

none

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-SLC35A1-GFP

Plasmid

#186281

Purpose

transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.L

Plasmid

#65414

Purpose

contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Article

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

TNC large splice variant (TNC Human)

Expression

Bacterial

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-Cdc42

Plasmid

#12201

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 19

Plasmid

#134987

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Article

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Insert

Myo 19 (MYO19 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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SIVl

Plasmid

#81090

Purpose

Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRAv3:minP-GFP

Plasmid

#109036

Purpose

MPRA v3 minimal promoter with GFP donor

Depositor

Pardis Sabeti, Ryan Tewhey

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Insert

emGFP

Expression

Mammalian

Promoter

minP

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5CA (Q79L)

Plasmid

#35140

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS sfGFP

Plasmid

#154462

Purpose

pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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SIVt

Plasmid

#81092

Purpose

Single Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSelAct-KO

Plasmid

#174374

Purpose

Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

Depositor

Gitta Coaker

Article

Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

Type

Empty backbone

Use

Vector for 2-step knockout by homologous recombin…

Promoter

N/A

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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SIVh

Plasmid

#81091

Purpose

Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ14103 aeBlue-targeting_sgRNA

Plasmid

#239750

Purpose

guide for aeBlue

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting aeBlue

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hTDG

Plasmid

#70758

Purpose

bacterial expression of human TDG

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

TDG (TDG Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MCP-EGFP-PiggyBac

Plasmid

#190003

Purpose

Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-EGFP

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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